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DSMZ

DSMZ 1323 . METHYLOCAPSA AUREA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1323 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 200 mg 0.2 2.353
MgSO4 x 6 H2O 200 mg 0.2 1.662
CaCl2 x 2 H2O 40 mg 0.04 0.272
FeEDTA solution 1 ml - -
100mM Phosphate buffer 10 ml - -
Trace elements 1 ml - -
Distilled water 986 ml - -
1 Final pH 5.0-5.8.
2 20-30% methane is added to the gas phase. The strain should be grown with shaking.

Trace elements #

1000
Solution occurs in 1 other media (first defined in Medium 1322).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
CuCl2 x 5 H2O 0.10 g 0.1 0.445
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
NiCl2 x 6 H2O 0.02 g 0.02 0.154
CoCl2 x 6 H2O 0.20 g 0.2 1.54
Na2MoO4 0.03 g 0.03 0.146
Distilled water 1000.00 ml - -

FeEDTA solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 1.54 g 1.54 5.539
Na2-EDTA 2.06 g 2.06 6.127
Distilled water 1000.00 ml - -

100mM Phosphate buffer #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 x 3 H2O 1.6 g 1.71 9.818
KH2PO4 12.5 g 12.58 92.442
Distilled water 1000.0 ml - -
1 pH 5.8

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 5.0 - 5.8
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Gas composition

  • 25 % CH4

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaNO3 0.2 2.353
MgSO4 x 6 H2O 0.2 1.662
KH2PO4 0.126 0.924
CaCl2 x 2 H2O 0.04 0.272
K2HPO4 x 3 H2O 0.017 0.098
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
temperature mesophilic 1 strain