1331
1331 .
COLUMBIA AGAR WITH 5% SHEEP BLOOD
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1331 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Columbia Blood Agar Base | 44 | g | - | - | |
| Distilled water | 1000 | ml | - | - | |
| Defibrinated Blood | 50 | ml | - | - | |
| 1 1. Suspend 44 g Columbia Blood Agar Base in 1l deionized water. Heat to boiling to dissolve completely. | |||||
| 2 2. Sterilize by autoclaving for 15 min at 121°C. | |||||
| 3 3. Cool to 45°C. Add 5% sterile defibrinated sheep blood and mix well. | |||||
| 4 Rehydrate pellets of freeze-dried ampoules in liquid medium 220 (see below). | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
Bacto peptone
(Peptone) |
10 | g | 9.524 | - |
|
Bacto peptone
(Peptone, Bitone) |
10 | g | 9.524 | - |
| Tryptic Digest of beef heart | 3 | g | 2.857 | - |
| Corn starch | 1 | g | 0.952 | - |
| NaCl | 5 | g | 4.762 | 81.484 |
| Agar | 15 | g | 14.286 | 42.475 |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 15 | g | 15 | - | |
| Soy peptone | 5 | g | 5 | - | |
| NaCl | 5 | g | 5 | 85.558 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid Cm131). | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | 7.3 |
| Edit in Medium builder | |