1332 .
ALGORIPHAGUS AQUAEDUCTUS MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
8.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Meat extract | 20.00 | g | ||
|
|
NH4Cl | 1.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.20 | g | ||
|
|
CaCl2 x 2 H2O | 0.01 | g | ||
|
|
KH2PO4 | 0.50 | g | ||
|
|
Seven vitamins solution | 3.00 | ml | ||
|
|
Trace element solution SL-8 | 1.00 | ml | ||
|
|
Distilled water | 996.00 | ml | ||
|
|
Agar , for solid medium | 15.00 | g | ||
| 1 Adjust pH to 8.0 before autoclaving. The medium may be solidified by adding 15 g/l agar. | |||||
Trace element solution SL-8 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 5.2 | g | ||
|
|
FeSO4 x 7 H2O | 2.0 | g | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
MnCl2 x 2 H2O | 100.0 | mg | ||
|
|
ZnSO4 x 7 H2O | 150.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
H3BO3 | 62.0 | mg | ||
|
|
CuCl2 x 2 H2O | 17.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 6.8. | |||||
Seven vitamins solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Vitamin B12 | 100 | mg | ||
|
|
p-Aminobenzoic acid | 80 | mg | ||
|
|
D-(+)-biotin | 20 | mg | ||
|
|
Nicotinic acid | 200 | mg | ||
|
|
Calcium pantothenate | 100 | mg | ||
|
|
Pyridoxine hydrochloride | 300 | mg | ||
|
|
Thiamine-HCl x 2 H2O | 200 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Filter sterilise and add to the medium after it has been autoclaved. | |||||