1338
1338 .
LB MEDIUM WITH CARBONATES
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1338 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Mineral Salts Solution | 500 | ml | - | - | |
| Carbonate Salts Solution | 500 | ml | - | - | |
| 1 Autoclave the Mineral Salts and the Carbonate Salts separately. Combine both solutions after cooling. The medium may be solidified by adding 20.0 g/l agar. The agar is added to Minerals Salts Solution and after autoclaving both the Mineral Salts Solution and the Carbonate Salts Solution are cooled to 50-55°C, mixed and poured into Petri dishes. Final pH 9.6. | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaHCO3 | 5 | g | 10 | 119.038 |
| Na2CO3 | 5 | g | 10 | 94.35 |
| Distilled water | 500 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Tryptone | 10 | g | 20 | 281.373 |
| Yeast extract | 5 | g | 10 | - |
| NaCl | 10 | g | 20 | 342.231 |
| Distilled water | 500 | ml | - | - |
| Agar , for solid medium | 20 | g | 40 | 118.931 |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.6 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 20 | 59.466 |
| NaCl | 10 | 171.115 |
| Tryptone | 10 | 140.686 |
| NaHCO3 | 5 | 59.519 |
| Na2CO3 | 5 | 47.175 |
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