1353 .
YTBC - MEDIUM
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Equipment needed:
Hungate tubes
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 1.00 | g | ||
|
|
Tryptone | 5.00 | g | ||
|
|
Casamino acids | 5.00 | g | ||
|
|
Beef extract
(Lab-Lemco-Powder) |
5.00 | g | ||
|
|
K2HPO4 | 1.00 | g | ||
|
|
NH4NO3 | 1.00 | g | ||
|
|
Na2S x 9 H2O | 0.25 | g | ||
|
|
Cysteine HCl | 0.25 | g | ||
|
|
NiCl2 x 6 H2O | 7.50 | mg | ||
|
|
Trace element solution | 5.00 | ml | ||
|
|
Resazurin
(0.1% v/v) |
0.50 | ml | ||
|
|
Artifical seawater solution | 250.00 | ml | ||
|
|
Distilled water | 750.00 | ml | ||
| 1 Prepare the liquid medium without Na2S and cysteine-HCl. Boil the medium in a water bath and cool under N2:CO2 (80:20 v/v) to room temperature. Adjust to pH 7.0 with 10 M NaOH solution. Dispense the medium into Hungate tubes or serum bottles. Sterilize by autoclaving. Before inoculatiing add Na2S and cysteine-HCl from sterile stock solutions. | |||||
Trace element solution #
500
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FePO4 | 1.00 | g |
|
|
Na2WO4 x 2 H2O | 0.01 | g |
|
|
Na2SO3 x 5 H2O | 0.01 | g |
|
|
Distilled water | 500.00 | ml |
Artifical seawater solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaCl | 20.00 | g |
|
|
MgCl2 x 6 H2O | 3.00 | g |
|
|
MgSO4 x 7 H2O | 6.00 | g |
|
|
NaHCO3 | 0.20 | g |
|
|
CaCl2 x 2 H2O | 0.30 | g |
|
|
KCl | 0.50 | g |
|
|
KH2PO4 | 0.42 | g |
|
|
NaBr | 0.05 | g |
|
|
SrCl2 | 0.02 | g |
|
|
Fe(NH4)citrate | 0.01 | g |
|
|
(NH4)2SO4 | 1.00 | g |
|
|
Distilled water | 1000.00 | ml |