Name: THIORHODOCOCCUS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1358 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.50	g	0.5	3.674
NaCl	10.00	g	10	171.116
NH₄Cl	0.60	g	0.6	11.217
CaCl₂ x 2 H₂O	0.15	g	0.15	1.02
MgSO₄ x 7 H₂O	2.00	g	2	8.114
Na pyruvate	3.00	g	3	27.262
NaHCO₃ (10.0% w/v)	5.00	ml	0.5	5.952
Na₂S x 9 H₂O (24% w/v)	1.00	ml	0.24	0.999
Na₂S₂O₃ x 5 H₂O (24 % w/v)	5.00	ml	1.2	4.835
Yeast extract	0.30	g	0.3	-
Micronutrient solution SL8	1.00	ml	-	-
Vitamin B₁₂ (20mg/l)	1.00	ml	-	-
Distilled water	1000.00	ml	-	-
1 Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas.
2 Prepare the medium without the NaHCO₃, Na₂S x 9 H₂O, Na₂S2O3 x 5 H₂O and vitamin B₁₂, under a nitrogen atmosphere dispense into tubes or bottles fitted with rubber stoppers so that they are half full. Autoclave and add the vitamin B₁₂ from a filter-sterilised stock solution and the NaHCO₃, Na₂S x 9 H₂O, Na₂S2O3 x 5 H₂O from sterile stock solutions. The final pH of the medium should be 7.5 + 0.2.

Micronutrient solution SL8 #

1000

Equipment needed: Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂-EDTA	5.2	g	5.2	15.467
FeCl₂ x 4 H₂O	1.5	g	1.5	7.545
ZnCl₂	70.0	mg	0.07	0.514
MnCl₂ x 4 H₂O	100.0	mg	0.1	0.505
H₃BO₃	62.0	mg	0.062	1.003
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
CuCl₂ x 2 H₂O	17.0	mg	0.017	0.1
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	1000.0	ml	-	-
1 Adjust to pH 6.5

All strains for this medium:	1
Strains with modifications:	1

1 strain needs light for growth. Select a strain for more details.

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