This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.50 | g | 0.5 | 3.674 | |
NaCl | 10.00 | g | 10 | 171.116 | |
NH4Cl | 0.60 | g | 0.6 | 11.217 | |
CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.02 | |
MgSO4 x 7 H2O | 2.00 | g | 2 | 8.114 | |
Na pyruvate | 3.00 | g | 3 | 27.262 | |
NaHCO3
(10.0% w/v) |
5.00 | ml | 0.5 | 5.952 | |
Na2S x 9 H2O
(24% w/v) |
1.00 | ml | 0.24 | 0.999 | |
Na2S2O3 x 5 H2O
(24 % w/v) |
5.00 | ml | 1.2 | 4.835 | |
Yeast extract | 0.30 | g | 0.3 | - | |
Micronutrient solution SL8 | 1.00 | ml | - | - | |
Vitamin B12
(20mg/l) |
1.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. | |||||
2 Prepare the medium without the NaHCO3, Na2S x 9 H2O, Na2S2O3 x 5 H2O and vitamin B12, under a nitrogen atmosphere dispense into tubes or bottles fitted with rubber stoppers so that they are half full. Autoclave and add the vitamin B12 from a filter-sterilised stock solution and the NaHCO3, Na2S x 9 H2O, Na2S2O3 x 5 H2O from sterile stock solutions. The final pH of the medium should be 7.5 + 0.2. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 5.2 | g | 5.2 | 15.467 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 62.0 | mg | 0.062 | 1.003 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 17.0 | mg | 0.017 | 0.1 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 1000.0 | ml | - | - | |
1 Adjust to pH 6.5 |
Last modified: | 31.03.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.3 - 7.7 |
Equipment needed: | Autoclave, Filter |
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Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 10 | 171.116 |
Na pyruvate | 3 | 27.262 |
MgSO4 x 7 H2O | 2 | 8.114 |
Na2S2O3 x 5 H2O | 1.2 | 4.835 |
NH4Cl | 0.6 | 11.217 |
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