1359 .
PHAEOVIBRIO SULPHIDIPHILUS MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
6.6 - 7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.50 | g | ||
|
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
|
NaCl | 0.40 | g | ||
|
|
NH4Cl | 0.68 | g | ||
|
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
|
Na pyruvate | 0.50 | g | ||
|
|
Sodium succinate | 0.50 | g | ||
|
|
Na acetate | 0.50 | g | ||
|
|
Yeast extract | 0.50 | g | ||
|
|
Ferric citrate
(0.1%) |
5.00 | ml | ||
|
|
NaHCO3
(10%) |
15.00 | ml | ||
|
|
Na2S x 9 H2O
(1M) |
1.00 | ml | ||
|
|
Vitamin B12
(2mg/100ml) |
1.00 | ml | ||
|
|
Trace element solution SL7 | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Prepare the basal medium under nitrogen. | |||||
| 2 NaHCO3 solution (autoclaved in sealed, half full vessels under CO2), Na2S x 9H2O solution (autoclaved) and vitamin B12 solution (filter sterilised) is added to the autoclaved medium and the final pH adjusted to 6.6 - 7.4. | |||||
Trace element solution SL7 #
1001
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(25%, v/v) |
1 | ml |
|
|
ZnCl2 | 70 | mg |
|
|
MnCl2 x 4 H2O | 100 | mg |
|
|
H3BO3 | 60 | mg |
|
|
CoCl2 x 6 H2O | 200 | mg |
|
|
CuCl2 x 2 H2O | 20 | mg |
|
|
NiCl2 x 6 H2O | 20 | mg |
|
|
Na2MoO4 x 2 H2O | 40 | mg |
|
|
Distilled water | 1000 | ml |