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DSMZ

DSMZ 1361 . PLANCTOMYCETALES BACTERIUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Thermopirellula anaerolimosa DSM 24165

This strain uses the standard medium without modifications.

Growth conditions: 45 °C

Main sol. 1361 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.14 g 0.14 1.029
MgCl2 x 6 H2O 0.20 g 0.2 0.984
CaCl2 x 2 H2O 0.15 g 0.15 1.02
NH4Cl 0.54 g 0.54 10.095
Trace elements Solution 1.00 ml - -
Glucose 0.18 g 0.18 0.999
Yeast extract 0.20 g 0.2 -
Vitamin solution 1.00 ml - -
Resazurin
(0.1 v/v) 		0.50 ml - -
NaHCO3 2.50 g 2.5 29.759
Na2S x 9 H2O 0.30 g 0.3 1.249
Cysteine HCl 0.30 g 0.3 2.476
Distilled water 1000.00 ml - -
1 Prepare the medium without yeast extract, glucose, NaHCO3, cysteine hydrochloride, vitamin solution and Na2S x 9 H2O. Boil and then cool under a gas atmosphere of N2:CO2 (80:20 v/v). Dispense the medium into Hungate tubes or serum bottles under gassing with N2:CO2 (80:20 v/v) and add the NaHCO3. The final pH should be 7.0. Autoclave the medium and add from sterile stock solutions yeast extract, glucose, cysteine hydrochloride, vitamin solution and Na2S x 9 H2O.The final pH should be 7.0.

Vitamin solution #

1000
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace elements Solution #

1000
Equipment needed: Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		12.800 g 12.8 66.967
FeCl3 x 6 H2O 1.350 g 1.35 4.995
MnCl2 x 4 H2O 0.100 g 0.1 0.505
CoCl2 x 6 H2O 0.024 g 0.024 0.185
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 2 H2O 0.025 g 0.025 0.147
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.024 g 0.024 0.099
NiCl2 x 6 H2O 0.120 g 0.12 0.926
Na2SeO3 x 5 H2O 4.000 mg 0.004 0.015
Distilled water 1000.000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Thermopirellula anaerolimosa DSM 24165

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.5 29.759
NH4Cl 0.54 10.095
Cysteine HCl 0.3 2.476
Na2S x 9 H2O 0.3 1.249
MgCl2 x 6 H2O 0.2 0.984
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain