1370 .
RICH ORGANIC MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MgSO4 x 7 H2O | 0.3 | g | ||
|
|
KH2PO4 | 0.3 | g | ||
|
|
NH4Cl | 0.1 | g | ||
|
|
CaCl2 x 2 H2O | 1.0 | g | ||
|
|
Na-acetate | 1.0 | g | ||
|
|
Yeast extract | 0.5 | g | ||
|
|
Peptone | 0.5 | g | ||
|
|
Casamino acids | 0.5 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 5.9. Add NaOH (0.1 mol/l) 16ml/l after sterilisation from sterile stock solution to readjust pH to 6.8. | |||||
| 2 Add the following from sterile stock solutions to the autoclaved medium: | |||||
|
|
Trace elements solution N1 | 2.0 | ml | ||
|
|
Vitamin solution N1 | 2.0 | ml | ||
Vitamin solution N1 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nicotinic acid | 400 | mg | ||
|
|
Thiamine | 400 | mg | ||
|
|
Biotin | 80 | mg | ||
|
|
Vitamin B12 | 20 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Filter sterilised | |||||
Trace elements solution N1 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeSO4 x 7 H2O | 300 | mg |
|
|
ZnSO4 x 7 H2O | 5 | mg |
|
|
MnCl2 x 4 H2O | 3 | mg |
|
|
H3BO3 | 2 | mg |
|
|
CoCl2 x 6 H2O | 5 | mg |
|
|
CuCl2 x 2 H2O | 1 | mg |
|
|
NiCl2 x 6 H2O | 2 | mg |
|
|
Na2MoO4 x 2 H2O | 3 | mg |
|
|
Distilled water | 1000 | ml |