1378 .
MO-CFXI MEDIUM
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Selected strain: Pelolinea submarina DSM 23923
This strain uses the standard medium without modifications.
Growth conditions: more than 14 days
anaerobic
25 °C
Equipment needed:
Autoclave
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 25.000 | g | ||
|
|
MgCl2 x 6 H2O | 4.000 | g | ||
|
|
KCl | 0.500 | g | ||
|
|
CaCl2 x 2 H2O | 1.000 | g | ||
|
|
NH4Cl | 0.535 | g | ||
|
|
KH2PO4 | 0.100 | g | ||
|
|
Tryptone | 1.000 | g | ||
|
|
Glucose | 1.800 | g | ||
|
|
Sodium pyruvate | 0.550 | g | ||
|
|
Yeast extract | 1.000 | g | ||
|
|
NaHCO3 | 2.000 | g | ||
|
|
Cysteine-HCl | 0.300 | g | ||
|
|
Resazurin
(1mg/ml) |
1.000 | ml | ||
|
|
Trace element solution | 1.000 | ml | ||
|
|
Distilled water | 1000.000 | ml | ||
|
|
Na2S | 0.030 | % | ||
|
|
Vitamin solution | 2.000 | ml | ||
| 1 pH 7.0 | |||||
| 2 Prepare under N2/CO2 (80/20) gas. | |||||
| 3 Autoclave and add the following components after autoclaving from stock solutions: | |||||
| 4 sterile Na2S solution end concentration 0.03% | |||||
| 5 and filter-sterile Vitamin solution (see below) 0.2 ml / 100 ml. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeCl2 x 4 H2O | 1.27 | g |
|
|
ZnCl2 | 0.14 | g |
|
|
MnCl2 x 4 H2O | 0.20 | g |
|
|
Na2SeO4 | 2.00 | mg |
|
|
H3BO3 | 6.00 | mg |
|
|
Na2WO4 x 2 H2O | 3.00 | mg |
|
|
CuCl2 x 2 H2O | 1.00 | mg |
|
|
CoCl2 x 6 H2O | 0.13 | g |
|
|
AlCl3 | 0.01 | g |
|
|
NiCl2 | 0.01 | g |
|
|
Na2MoO4 x 2 H2O | 0.02 | g |
|
|
Distilled water | 1000.00 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 4.9 | mg |
|
|
Folic acid | 8.8 | mg |
|
|
Pyridoxine hydrochloride | 4.1 | mg |
|
|
Thiamine-HCl x 2 H2O | 6.7 | mg |
|
|
Riboflavin | 7.5 | mg |
|
|
Nicotinic acid | 2.4 | mg |
|
|
D-Calcium pantothenate | 9.5 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 2.7 | mg |
|
|
Lipoic acid | 4.1 | mg |
|
|
Distilled water | 1000.0 | ml |