1378
1378 .
MO-CFXI MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Pelolinea submarina DSM 23923
This strain uses the standard medium without modifications.
Growth conditions: more than 14 days
anaerobic
25 °C
Main sol. 1378 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 25.000 | g | 25 | 427.789 | |
| MgCl2 x 6 H2O | 4.000 | g | 4 | 19.675 | |
| KCl | 0.500 | g | 0.5 | 6.707 | |
| CaCl2 x 2 H2O | 1.000 | g | 1 | 6.802 | |
| NH4Cl | 0.535 | g | 0.535 | 10.002 | |
| KH2PO4 | 0.100 | g | 0.1 | 0.735 | |
| Tryptone | 1.000 | g | 1 | 14.069 | |
| Glucose | 1.800 | g | 1.8 | 9.991 | |
| Sodium pyruvate | 0.550 | g | 0.55 | 4.998 | |
| Yeast extract | 1.000 | g | 1 | - | |
| NaHCO3 | 2.000 | g | 2 | 23.808 | |
| Cysteine-HCl | 0.300 | g | 0.3 | 1.903 | |
|
Resazurin
(1mg/ml) |
1.000 | ml | - | - | |
| Trace element solution | 1.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| Na2S | 0.030 | % | 0.3 | 3.795 | |
| Vitamin solution | 2.000 | ml | - | - | |
| 1 pH 7.0 | |||||
| 2 Prepare under N2/CO2 (80/20) gas. | |||||
| 3 Autoclave and add the following components after autoclaving from stock solutions: | |||||
| 4 sterile Na2S solution end concentration 0.03% | |||||
| 5 and filter-sterile Vitamin solution (see below) 0.2 ml / 100 ml. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeCl2 x 4 H2O | 1.27 | g | 1.27 | 6.388 |
| ZnCl2 | 0.14 | g | 0.14 | 1.027 |
| MnCl2 x 4 H2O | 0.20 | g | 0.2 | 1.011 |
| Na2SeO4 | 2.00 | mg | 0.002 | 0.011 |
| H3BO3 | 6.00 | mg | 0.006 | 0.097 |
| Na2WO4 x 2 H2O | 3.00 | mg | 0.003 | 0.009 |
| CuCl2 x 2 H2O | 1.00 | mg | 1.0e-3 | 0.006 |
| CoCl2 x 6 H2O | 0.13 | g | 0.13 | 1.001 |
| AlCl3 | 0.01 | g | 0.01 | 0.075 |
| NiCl2 | 0.01 | g | 0.01 | 0.077 |
| Na2MoO4 x 2 H2O | 0.02 | g | 0.02 | 0.083 |
| Distilled water | 1000.00 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 4.9 | mg | 0.005 | 0.02 |
| Folic acid | 8.8 | mg | 0.009 | 0.02 |
| Pyridoxine hydrochloride | 4.1 | mg | 0.004 | 0.02 |
| Thiamine-HCl x 2 H2O | 6.7 | mg | 0.007 | 0.018 |
| Riboflavin | 7.5 | mg | 0.008 | 0.02 |
| Nicotinic acid | 2.4 | mg | 0.002 | 0.019 |
| D-Calcium pantothenate | 9.5 | mg | 0.01 | - |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 2.7 | mg | 0.003 | 0.02 |
| Lipoic acid | 4.1 | mg | 0.004 | 0.02 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
| Strains with modifications: | 1 |
Pelolinea submarina DSM 23923
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 25 | 427.789 |
| MgCl2 x 6 H2O | 4 | 19.675 |
| NaHCO3 | 2 | 23.808 |
| Glucose | 1.8 | 9.991 |
| Tryptone | 1 | 14.069 |
| Show more | ||
