1383 .
VL55 MEDIUM modified
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Equipment needed:
Autoclave
Filter
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MES
(2-Morpholinoethanesulfonic acid) |
1.95 | g | ||
|
|
MgSO4 x 7 H2O
(20mM) |
10.00 | g | ||
|
|
CaCl2 x 2 H2O
(30mM) |
10.00 | g | ||
|
|
(NH4)2HPO4
(20mM) |
10.00 | g | ||
|
|
Selenite-tungstate solution | 1.00 | ml | ||
|
|
Trace element solution SL-10 | 1.00 | ml | ||
|
|
Distilled water | 940.00 | ml | ||
|
|
Vitamin solution | 3.00 | ml | ||
|
|
Glucose | 2.00 | mM | ||
| 1 Adjust to pH 5.5 with NaOH/KOH (about 6 ml). | |||||
| 2 Autoclave and add 3 ml/l vitamin solution (see Medium1266) and 2mM Glucose end-concentration. | |||||
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Distilled water | 1000.0 | ml |
|
|
NaOH | 0.5 | g |
|
|
Na2SeO3 x 5 H2O | 3.0 | mg |
|
|
Na2WO4 x 2 H2O | 4.0 | mg |
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(25%; 7.7 M) |
10.0 | ml |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
ZnCl2 | 70.0 | mg |
|
|
MnCl2 x 4 H2O | 100.0 | mg |
|
|
H3BO3 | 6.0 | mg |
|
|
CoCl2 x 6 H2O | 190.0 | mg |
|
|
CuCl2 x 2 H2O | 2.0 | mg |
|
|
NiCl2 x 6 H2O | 24.0 | mg |
|
|
Na2MoO4 x 2 H2O | 36.0 | mg |
|
|
Distilled water | 990.0 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Vitamin B12 | 17 | mg | ||
|
|
4-aminobenzoate | 13 | mg | ||
|
|
Biotin | 3 | mg | ||
|
|
Nicotinic acid | 33 | mg | ||
|
|
Hemicalcium D-(+)-pantothenate | 17 | mg | ||
|
|
Pyridoxamine hydrochloride | 50 | mg | ||
|
|
Thiamine-HCl x 2 H2O | 33 | mg | ||
|
|
DL-6,8-thioctic acid | 10 | mg | ||
|
|
Riboflavin | 10 | mg | ||
|
|
Folic acid | 4 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Filter sterilize the solution. | |||||