1398 .
1/10 PYGV MEDIUM modified
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Equipment needed:
Autoclave
Filter
Hungate tubes
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
S I | 75.0 | ml | ||
|
|
S II | 75.0 | ml | ||
|
|
Trypticase | 1.0 | g | ||
|
|
Yeast extract | 0.5 | mg | ||
|
|
Glucose | 1.0 | g | ||
|
|
L-Cysteine HCl | 0.3 | mg | ||
|
|
Resazurin
(0.1%) |
0.5 | ml | ||
|
|
100x Vitamin solution | 10.0 | ml | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 SII | |||||
| 2 pH 7.0 | |||||
| 3 Prepare the medium without the vitamin solution under an atmosphere of N2 and dispense into Hungate tubes. Seal under an atmosphere of nitrogen with rubber stoppers and autoclave. Aseptically add the vitamin solution from a filter sterilised stock solution to the sterile medium. | |||||
100x Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 0.1 | g |
|
|
Cobalamine | 0.1 | g |
|
|
Thiamine-HCl x 2 H2O | 0.5 | g |
|
|
p-Aminobenzoic acid | 0.3 | g |
|
|
Pyridoxine | 1.5 | g |
|
|
Riboflavin | 0.5 | g |
|
|
Folic acid | 0.5 | g |
|
|
Distilled water | 1000.0 | ml |
S II #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
KH2PO4 | 6.0 | g |
|
|
(NH4)2SO4 | 12.0 | g |
|
|
NaCl | 12.0 | g |
|
|
MgSO4 x 7 H2O | 1.2 | g |
|
|
CaCl2 x 2 H2O | 1.2 | g |
|
|
Distilled water | 1000.0 | ml |