1414 .
PORPHYROBACTER MEDIUM
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Equipment needed:
Filter
Final pH:
8.0 - 8.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MgSO4 x 7 H2O | 2.0 | g | ||
|
|
NH4Cl | 0.3 | g | ||
|
|
KCl | 0.3 | g | ||
|
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Na2SO4 | 15.0 | g | ||
|
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Na acetate | 1.0 | g | ||
|
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Malic acid | 1.0 | g | ||
|
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Yeast extract | 1.0 | g | ||
|
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Bacto peptone | 0.5 | g | ||
|
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Trace Element Solution | 2.0 | ml | ||
|
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Vitamine Solution | 2.0 | ml | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust to pH 5.9 before autoclaving | |||||
| 2 Prepare the liquid medium. Before inoculation add from sterile stock solutions: | |||||
|
|
NaHCO3
(10% w/v) |
5.0 | ml/l | ||
|
|
CaCl2
(10% w/v) |
20.0 | ml/l | ||
|
|
KH2PO4
(10% w/v) |
3.0 | ml/l | ||
|
|
Vitamine Solution | 2.0 | ml/l | ||
| 3 Adjust to pH 8.0 - 8.5 with 0.5 N NaOH. | |||||
Trace Element Solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
FeSO4 x 7 H2O | 300 | mg | ||
|
|
MnCl2 x 4 H2O | 3 | mg | ||
|
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CoCl2 x 6 H2O | 5 | mg | ||
|
|
CuCl2 x 2 H2O | 1 | mg | ||
|
|
NiCl2 x 6 H2O | 2 | mg | ||
|
|
Na2MoO4 x 2 H2O | 3 | mg | ||
|
|
ZnSO4 x 7 H2O | 5 | mg | ||
|
|
H3BO3 | 2 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust to pH 3.0 | |||||
Vitamine Solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nicotinic acid | 400 | mg | ||
|
|
Thiamine | 400 | mg | ||
|
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Biotine | 80 | mg | ||
|
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Vitamin B12 | 20 | mg | ||
|
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Distilled water | 1000 | ml | ||
| 1 Filtersterilized | |||||