1417 .
MMB MEDIUM
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Equipment needed:
Autoclave
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 0.15 | g | ||
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Yeast extract | 0.15 | g | ||
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Ammonium Sulfate | 0.25 | g | ||
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Glucose | 1.00 | g | ||
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Modified Hutner`s Basal Salts Solution | 20.00 | ml | ||
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Distilled water | 1000.00 | ml | ||
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Agar , for solid medium | 15.00 | g | ||
| 1 Autoclave at 121 °C for 15 min. | |||||
|
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Vitamine Solution | 10.00 | ml | ||
Vitamine Solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
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Folic acid | 2.0 | mg |
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Thiamine HCl | 5.0 | mg |
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D-Calcium pantothenate | 5.0 | mg |
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Vitamin B12 | 0.1 | mg |
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Riboflavin | 5.0 | mg |
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Distilled water | 1000.0 | ml |
Modified Hutner`s Basal Salts Solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 10.00 | g | ||
|
|
MgSO4 x 7 H2O | 29.70 | g | ||
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CaCl2 x 2 H2O | 3.34 | g | ||
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Ammonium Molybdate | 9.25 | mg | ||
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FeSO4 x 7 H2O | 99.00 | mg | ||
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Metals "44" | 50.00 | ml | ||
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Distilled water | 950.00 | ml | ||
| 1 Note: Dissolve and neutralize the nitrilotriacetic acid with KOH (7.3 g); add the other ingredients and adjust the pH to 6.6 - 6.8 before bringing the volume to 1.0 L with distilled water. | |||||
Metals "44" #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 0.250 | g | ||
|
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ZnSO4 x 7 H2O | 1.100 | g | ||
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FeSO4 x 7 H2O | 0.500 | g | ||
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MnSO4 x 7 H2O | 0.154 | g | ||
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CuSO4 x 5 H2O | 0.040 | g | ||
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Co(NO3)2 x 6 H2O | 0.025 | g | ||
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Na2B4O7 x 10 H2O | 0.018 | g | ||
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Distilled water | 1000.000 | ml | ||
| 1 Note: Initially add a few drops of H2SO4 to the distilled water to retard precipitation. | |||||