1431 .
ALKALOPHILIC HALOPHILE MEDIUM
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Equipment needed:
Autoclave
Final pH:
9.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 10.00 | g | ||
|
|
Casamino acids | 7.50 | g | ||
|
|
Trisodium citrate | 3.00 | g | ||
|
|
KCl | 2.00 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
MnCl2 x 4 H2O | 0.36 | mg | ||
|
|
FeSO4 x 7 H2O | 50.00 | mg | ||
|
|
NaCl | 200.00 | g | ||
|
|
Na2CO3 | 50.00 | g | ||
|
|
Agar | 2.00 | % | ||
| 1 Liquid medium may be prepared by adding all components together. Agar (2%) medium should be prepared without Na2CO3 and sterilised by autoclaving. Add 20-30 ml of a sterile 10% Na2CO3 solution to the autoclaved medium containing agar so that the final pH is 9.0. | |||||