1454 .
Meiothermus hypogaeus MEDIUM
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Equipment needed:
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract
(Difco, Bacto ) |
1.00 | g | ||
|
|
Bacto Tryptone
(Difco) |
1.00 | g | ||
|
|
Sodium glutamate monohydrate | 1.00 | g | ||
|
|
CaSO4 x 2 H2O | 0.06 | g | ||
|
|
MgSO4 x 7 H2O | 0.10 | g | ||
|
|
NaCl | 8.00 | mg | ||
|
|
KNO3 | 0.10 | g | ||
|
|
NaNO3 | 0.69 | g | ||
|
|
Na2HPO4 | 0.11 | g | ||
|
|
Trace element solution | 10.00 | ml | ||
|
|
Agar | 20.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.5 with NaOH. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid
(NTA) |
12.80 | g | ||
|
|
FeCl3 x 6 H2O | 1.35 | g | ||
|
|
MnCl2 x 4 H2O | 0.10 | g | ||
|
|
CoCl2 x 6 H2O | 24.00 | mg | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
|
ZnCl2 | 0.10 | g | ||
|
|
CuCl2 x 2 H2O | 25.00 | mg | ||
|
|
H3BO3 | 0.01 | g | ||
|
|
Na2MoO4 x 2 H2O | 24.00 | mg | ||
|
|
NaCl | 1.00 | g | ||
|
|
NiCl2 x 6 H2O | 0.12 | g | ||
|
|
Na2SeO4 | 4.00 | mg | ||
|
|
Na2WO4 | 4.00 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 First dissolve the nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0. | |||||