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DSMZ

DSMZ 1457 . ALCALILIMNICOLA EHRLICHII MHLE-1 MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Alkalilimnicola ehrlichii DSM 17681

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 1457 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 496 ml - -
Solution B 500 ml - -
Solution C 10 ml - -
Agar , for solid medium 20 g 19.881 59.111
1 Autoclave solution A without the vitamin solution. Autoclave solution B in half full, closed bottles. Autoclave solution C. Combine the sterile solutions A, B, and C after cooling. Aseptically add 1.0 ml of the vitamin solution (filter sterilized) to 1 litre of the sterile medium.
2 Adjust to pH 9.8. The medium may be solidified by adding 20 g/l agar, but care should be taken that the temperature of the solutions to be mixed are about 50°C, otherwise the agar will darken and degrade.

Solution A #

496
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.10 g 0.202 1.526
MgSO4 25.00 mg 0.05 0.419
NaCl 60.00 g 120.968 2069.95
K2HPO4 0.15 g 0.302 1.736
KH2PO4 0.08 g 0.161 1.185
Trace element solution SL-10 5.00 ml - -
Tungstate
(0.1 mM) 		0.30 ml 7.4e-6 3.0e-5
Vitamin solution 1.00 ml - -
Distilled water 490.00 ml - -

Solution B #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 10.6 g 21.2 200.023
NaHCO3 4.2 g 8.4 99.992
Distilled water 500.0 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-acetate
(1M) 		10 ml 0.82 10

Vitamin solution #

1000
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace element solution SL-10 #

1000
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%; 7.7 M) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Make up to a final volume of 1000.0 ml.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 9.8
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1
Alkalilimnicola ehrlichii DSM 17681

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 59.642 1020.57
Agar 19.881 59.111
Na2CO3 10.537 99.415
NaHCO3 4.175 49.698
K2HPO4 0.149 0.856
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 1 strain