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DSMZ

DSMZ 1469 . THERMOCHROMATIUM/ALLOCHROMATIUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1469 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 Mineral Salts 700.000 ml - -
Solution 2 NaHCO3 300.000 ml - -
1 Prepare solution 1 (mineral salts)
2 Prepare solution 2
3 Combine solutions 1 + 2. If desired aliquot into hungate tubes, filling them up fully.
4 Autoclave at 121 °C for 15 min.
5 Before inoculation add the following from anaerobic, sterile stock solutions.
Vitamine B12 40.000 μg/l - -
Yeast extract 0.010 % 0.1 -
Sodium pyruvate 0.050 % 0.5 4.544
Na2S x 9 H2O
(from neutralized stock solution, pH 7) 		0.075 % 0.75 3.123
6 check pH and adjust to 7.0 if necessary

Solution 1 Mineral Salts #

600
Compound Amount Unit Conc. [g/L] Conc. [mM]
Double distilled water 700.000 ml - -
EDTA
(1% stock solution) 		1.000 ml 0.017 0.057
MgSO4 x 7 H2O
(20% stock solution) 		1.000 ml 0.333 1.352
CaCl2 x 2 H2O
(7.5% stock solution) 		0.075 g 0.125 0.85
KH2PO4
(monobasic) 		0.500 g 0.833 6.124
NaCl 0.400 g 0.667 11.408
NH4Cl 0.400 g 0.667 12.463
Na acetate 1.000 g 1.667 20.317
Trace elements 1.000 ml - -
MOPS
(5 mM) 		1.050 g 1.75 8.363
1 Autoclave at 121°C for 15 min
2 Adjust pH to 7.0.
3 allow to cool before combining with solution 2

Solution 2 NaHCO3 #

300
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 1 g 3.333 39.679
Deionized water 300 ml - -
1 Boil briefly and bubble with CO2 for 30 minutes and filter sterilize into a sterile, gas-tight screw-cap bottle.

Solution 3 Na2S x 9H2O #

100
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.75 g 7.5 31.226
Water
(degassed) 		100.00 ml - -
1 Degas water by bringing to a boil for a minute or two. Meanwhile, wash sulfide before weighing – using plastic-forceps, dip a small chunk into dd H2O and dry sulfide and forceps with lint-free wipes. Drop weighed sulfide chunk into the degassed water.
2 Autoclave at 121 °C for 15 min.
3 Adjust pH to 7.0.

Trace elements #

1000
Solution occurs in 2 other media (first defined in Medium 1147).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.2 g 5.2 17.794
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
MnCl2 x 4 H2O 0.1 g 0.1 0.505
FeCl2 x 4 H2O 1.5 g 1.5 7.545
H3BO3 6.0 mg 0.006 0.097
CuCl2 x 2 H2O 17.0 mg 0.017 0.1
Na2MoO4 x 2 H2O 188.0 mg 0.188 0.777
NiCl2 x 6 H2O 25.0 mg 0.025 0.193
ZnCl2 70.0 mg 0.07 0.514
VOSO4 x 2 H2O 30.0 mg 0.03 0.151
Na2WO4 x 2 H2O 2.0 mg 0.002 0.006
NaHSeO3 2.0 mg 0.002 -
Deionized water 1000.0 ml - -

Metadata

Last modified: 06.11.23
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MOPS 1.225 5.854
Na acetate 1.167 14.222
NaHCO3 1 11.904
Na2S x 9 H2O 0.75 3.123
KH2PO4 0.583 4.287
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 1 strain
temperature thermophilic 2 strains