1493 .
VERRUCOMICROBIA MEDIUM
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Equipment needed:
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaHCO3 | 0.50 | g | ||
|
|
NaCl | 0.50 | g | ||
|
|
KH2PO4 | 0.40 | g | ||
|
|
(NH4)2SO4 | 0.20 | g | ||
|
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MgSO4 x 6 H2O | 0.10 | g | ||
|
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CaCl2 | 0.01 | g | ||
|
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Li2B4O7 | 0.01 | g | ||
|
|
FeEDTA solution | 1.00 | ml | ||
|
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Trace element solution | 1.00 | ml | ||
|
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Distilled water | 1000.00 | ml | ||
| 1 Do not adjust the pH. | |||||
| 2 For plates add 8g/l Gelrite or 15g Phytagel and 1g/L MgCl. A carbon source is not required because the organism degrades the polysaccharides in Gelrite or Phytagel | |||||
| 3 For liquid medium use 0.8-2.0g/l Gelrite as carbon source (the medium remains liquid). Alternatively mannose (1g/l) may be used instead of Gelrite in liquid medium. Do not add mannose to solid media, which will inhibit growth. | |||||
FeEDTA solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeSO4 x 7 H2O | 1.54 | g |
|
|
Na-EDTA | 2.06 | g |
|
|
Distilled water | 1000.00 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.500 | g | ||
|
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Fe(NH4)2(SO4)2 x 6 H2O | 0.200 | g | ||
|
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Na2SeO4 x 10 H2O | 0.440 | g | ||
|
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CoCl2 x 6 H2O | 0.180 | g | ||
|
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MnSO4 x H2O | 0.120 | g | ||
|
|
Na2MoO4 | 0.160 | g | ||
|
|
Na2WO4 | 0.100 | g | ||
|
|
ZnSO4 x 7 H2O | 0.540 | g | ||
|
|
AlCl3 | 0.040 | g | ||
|
|
NiCl2 x 6 H2O | 0.025 | g | ||
|
|
H3BO3 | 0.110 | g | ||
|
|
CuSO4 | 0.210 | g | ||
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MnCl2 | 0.190 | g | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. | |||||
| 2 Final pH 7.0 (with KOH). | |||||