1495
1495 .
AQUASPIRILLUM MEDIUM II
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1495 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Peptone
(BD) |
2.00 | g | 2 | - | |
| Succinic acid | 1.00 | g | 1 | 8.468 | |
| (NH4)2SO4 | 1.00 | g | 1 | 7.568 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| CaCl2 x 2 H2O | 0.03 | g | 0.03 | 0.204 | |
| Vitamin solution | 1.00 | ml | - | - | |
| Trace elements solution (Pfennig & Lippert, 1966) | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| Vitamin solution | 1.00 | ml | - | - | |
| Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
| 1 Adjust pH to 7.4-7.6 Autoclave and add 1.0 ml/l vitamin solution from a filter sterilised stock solution (see below). The medium may be solidified by adding 15.0 g/l agar. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 | |
| FeSO4 x 6 H2O | 2.20 | g | 2.2 | 14.482 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
| H3BO3 | 0.03 | g | 0.03 | 0.485 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 2 H2O | 0.03 | g | 0.03 | 0.176 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 pH 3.0-4.0 | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Biotin | 200 | mg | 0.2 | 0.819 | |
| Folic acid | 20 | mg | 0.02 | 0.045 | |
| Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 | |
| Thiamine HCl | 50 | mg | 0.05 | 0.148 | |
| Riboflavin | 100 | mg | 0.1 | 0.266 | |
| Nicotinic acid | 50 | mg | 0.05 | 0.406 | |
| DL-Pantothenic acid | 50 | mg | 0.05 | 0.228 | |
| Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 | |
| Para-aminobenzoic acid | 50 | mg | 0.05 | 0.365 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Filter sterilise. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.4 - 7.6 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 5 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Peptone | 2 | - |
| Succinic acid | 1 | 8.468 |
| (NH4)2SO4 | 1 | 7.568 |
| MgSO4 x 7 H2O | 1 | 4.057 |
| Show more | ||
