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DSMZ

DSMZ 1498 . PE MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1498 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na glutamate 0.5 g 0.5 2.957
Sodium succinate 0.5 g 0.5 3.085
Sodium acetate 0.5 g 0.5 6.095
Sodium Thiosulfate 0.5 g 0.5 3.162
Yeast extract
(BD) 		0.5 g 0.5 -
Casamino acids
(BD) 		0.5 g 0.5 -
(NH4)2SO4 0.5 g 0.5 3.784
Basal Salts Solution 5.0 ml - -
Phosphate solution (1M) 5.0 ml - -
Agar 15.0 g 15 44.599
Distilled water 1000.0 ml - -
Vitamin solution CA 1.0 ml - -
1 pH 7.5
2 Autoclave and add 1ml/l Vitamin solution (see Medium 87A)

Basal Salts Solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na3-EDTA 4.5320 g 4.532 12.653
FeSO4 x 7 H2O 1.1100 g 1.11 3.993
MgSO4 x 7 H2O 24.6500 g 24.65 100.01
CaCl2 x 2 H2O 2.9400 g 2.94 19.998
NaCl 23.4000 g 23.4 400.411
MnSO4 x 4 H2O 0.1116 g 0.112 0.5
ZnSO4 x 7 H2O 0.0288 g 0.029 0.1
Co(NO3)2 x 6 H2O 0.0292 g 0.029 0.1
CuSO4 x 6 H2O 0.0252 g 0.025 0.094
Na2MoO4 x 2 H2O 0.0242 g 0.024 0.1
H3BO3 0.0310 g 0.031 0.501
Distilled water 1000.0000 ml - -
1 Adjust to pH 7.0 with 10% KOH.

Phosphate solution (1M) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 136.09 g 136.09 1000.04
K2HPO4 228.23 g 228.23 1310.34
Distilled water 1000.00 ml - -
1 pH 7.2

Vitamin solution CA #

100
Solution occurs in 1 other media (first defined in Medium ).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 100 ml - -
Nicotinic acid 100 mg 1 8.123
Thiamine-HCl x 2 H2O 100 mg 1 2.679
Biotin 5 mg 0.05 0.205
p-Aminobenzoic acid 50 mg 0.5 3.646
Vitamin B12 1 mg 0.01 0.007
Calcium pantothenate 50 mg 0.5 1.049
Pyridoxine hydrochloride 50 mg 0.5 2.431
Folic acid 50 mg 0.5 1.133
Na3-EDTA 200 mg 2 5.584
1 Adjust pH to 7.5. The solution is filter-sterilized.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
K2HPO4 1.141 6.552
KH2PO4 0.68 5
Sodium acetate 0.5 6.095
(NH4)2SO4 0.5 3.784
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Cultivation metadata from 1 strains BacDive

phyla 2 2 strains