DSMZ 150a . ACIDITHIOBACILLUS CALDUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter Water bath
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 3.00 g 2.97 22.478
K2HPO4 x 3 H2O 0.50 g 0.495 2.842
MgSO4 x 7 H2O 0.50 g 0.495 2.009
KCl 0.10 g 0.099 1.328
Ca(NO3)2 x 4 H2O 0.02 g 0.02 0.084
Trace element solution 10.00 ml - -
Sulfur
(powdered)
5.00 g 4.951 154.365
Distilled water 1000.00 ml - -
1 Dissolve ingredients except trace elements and sulfur. Adjust pH with 6 N H2SO4 to 2.5, dispense into suitable cultivation vessels and autoclave. After autoclaving add filter-sterilized trace elements solution (see below) and sterile sulfur powder (sterilized by steaming for 3 hours on each of 3 successive days).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O 11.0 mg 1.1 4.07
CuSO4 x 5 H2O 0.5 mg 0.05 0.2
H3BO3 2.0 mg 0.2 3.234
MnSO4 x H2O 2.0 mg 0.2 1.183
Na2MoO4 x 2 H2O 0.8 mg 0.08 0.331
CoCl2 x 6 H2O 0.6 mg 0.06 0.462
ZnSO4 x 7 H2O 0.9 mg 0.09 0.313
Distilled water 10.0 ml - -

Metadata

Last modified: 13.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 2.5
Equipment needed: Autoclave, Filter, Water bath
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 4.951 154.365
(NH4)2SO4 2.97 22.478
K2HPO4 x 3 H2O 0.495 2.842
MgSO4 x 7 H2O 0.495 2.009
KCl 0.099 1.328
Show more

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain