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1515. OCEANIRHABDUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.0 g 0.996 7.012
2 30.0 g 29.881 511.28
3 0.7 g 0.697 9.352
4 5.0 g 4.98 24.496
5 1.0 g 0.996 6.775
6 0.4 g 0.398 2.287
7 0.4 g 0.398 2.928
8 0.5 g 0.498 9.31
9
(0.1% w/v)
2.0 ml 0.002 -
10 Trace element solution SL-10 1.0 ml - -
11
(OXOID)
3.0 g 2.988 -
12
(BD Bacto)
4.0 g 3.984 -
13 6.8 g 6.773 82.562
14
(0.1% w/v)
0.5 ml 5.0e-4 0.002
15 0.6 g 0.598 3.403
16 2.5 g 2.49 29.641
17 1000.0 ml 994.23 55188.1
Instructions:
  1. Dissolve ingredients except cysteine and bicarbonate, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve cysteine, adjust pH to 6.5, then dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.5 - 7.0, if necessary.
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.7
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 995.214 55242.7
NaCl 29.881 511.28
Sodium acetate 6.773 82.562
MgCl2 x 6 H2O 4.98 24.496
Tryptone 3.984 -
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Cultivation metadata from 1 strains BacDive

halophily moderate 1 strain
halophily not 1 strain
halophily slightly 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
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