1559 .
TRYPTONE-STARCH MEDIUM
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Equipment needed:
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Bacto Tryptone | 3.00 | g | ||
|
|
Starch
(soluble) |
2.00 | g | ||
|
|
HEPES | 2.38 | g | ||
|
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
|
Vitamin B12 | 0.50 | mg | ||
|
|
Agar
, for solid medium
(Difco) |
15.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Sterilize vitamin B12 separately by filtration. Adjust pH of medium to 7.0 with KOH prior to autoclaving. | |||||