1568
1568 .
MEDIUM FOR METHYLOCALDUM (RS11D-PR)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1568 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaNO3 | 1.00 | g | 1 | 11.765 | |
| MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.406 | |
| Na2HPO4 | 0.50 | g | 0.5 | 3.522 | |
| KH2PO4 | 0.22 | g | 0.22 | 1.617 | |
| CaCl2 x 2 H2O | 0.03 | g | 0.03 | 0.204 | |
| FeSO4 x 7 H2O | 2.00 | mg | 0.002 | 0.007 | |
| Trace elements | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Medium was adjusted pH 7.0 with NaOH | |||||
| 2 Dispense the medium into the growth vessels. If using sealed vessels it is appropriate to add 20% methane to the gas phase and autoclave at 121°C for 15 minutes. | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnSO4 x 7 H2O | 0.44 | g | 0.44 | 1.53 |
| CuSO4 x 5 H2O | 0.20 | g | 0.2 | 0.801 |
| MnSO4 x 2 H2O | 0.17 | g | 0.17 | 0.909 |
| Na2MoO4 | 0.06 | g | 0.06 | 0.291 |
| H3BO3 | 0.10 | g | 0.1 | 1.617 |
| CoCl2 x 6 H2O | 0.08 | g | 0.08 | 0.616 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Gas composition
- 20 % CH4
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaNO3 | 1 | 11.765 |
| Na2HPO4 | 0.5 | 3.522 |
| KH2PO4 | 0.22 | 1.617 |
| MgSO4 x 7 H2O | 0.1 | 0.406 |
| CaCl2 x 2 H2O | 0.03 | 0.204 |
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