DSMZ 1578 . 1/10 R2A MEDIUM

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Equipment needed: Autoclave

Final pH: 7.2

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ml
Compound Amount Unit
Yeast extract 0.05 g
Proteose peptone
(Difco no. 3)
0.05 g
Casamino acids 0.05 g
Glucose 0.05 g
Starch
(soluble)
0.05 g
Na-pyruvate 0.03 g
K2HPO4 0.03 g
MgSO4 x 7 H2O 5.00 mg
Agar 15.00 g
Distilled water 1000.00 ml
1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.