1586 .
ISP2 MODIFIED MEDIUM FOR METHYLIBIUM
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Equipment needed:
Filter
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract
(Difco) |
4 | g | ||
|
|
Malt extract
(Difco) |
10 | g | ||
|
|
Dextrose
(Difco) |
4 | g | ||
|
|
Trace element solution SL-10 | 1 | ml | ||
|
|
Agar | 20 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 pH 7.2 | |||||
| 2 Sterilize 20 min/121°C. After cooling to 60°C add to the medium: | |||||
|
|
Vitamin solution | 5 | ml | ||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(25%; 7.7 M) |
10.0 | ml |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
ZnCl2 | 70.0 | mg |
|
|
MnCl2 x 4 H2O | 100.0 | mg |
|
|
H3BO3 | 6.0 | mg |
|
|
CoCl2 x 6 H2O | 190.0 | mg |
|
|
CuCl2 x 2 H2O | 2.0 | mg |
|
|
NiCl2 x 6 H2O | 24.0 | mg |
|
|
Na2MoO4 x 2 H2O | 36.0 | mg |
|
|
Distilled water | 990.0 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Vitamin B12 | 0.2 | mg | ||
|
|
Biotin | 4.0 | mg | ||
|
|
Thiamine-HCl x 2 H2O | 10.0 | mg | ||
|
|
Calcium pantothenate | 10.0 | mg | ||
|
|
Folic acid | 1.0 | mg | ||
|
|
Riboflavin | 10.0 | mg | ||
|
|
Nicotinamide | 10.0 | mg | ||
|
|
p-Aminobenzoic acid | 10.0 | mg | ||
|
|
Pyridoxine hydrochloride | 10.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Filter-sterilized. | |||||