1608
1608 .
METHYLOPHILA MEDIUM (YIM 38)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1608 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(Bacto) |
4 | g | 4 | - | |
| Malt extract | 5 | g | 5 | - | |
|
Agar
(Bacto) |
15 | g | 15 | 44.599 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Sterilize 20 min./121°C. After cooling to 60°C add to the medium: | |||||
|
Glucose
(25%, sterile-filtered) |
16 | ml | 4 | 22.203 | |
| Vitamin solution | 5 | ml | - | - | |
| 2 Adjust pH to 7.5 | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Thiamine-HCl x 2 H2O
(B1) |
0.2 | mg | 2.0e-4 | 5.4e-4 | |
|
Pyridoxine hydrochloride
(B6) |
0.2 | mg | 2.0e-4 | 9.7e-4 | |
| Riboflavin | 0.2 | mg | 2.0e-4 | 5.3e-4 | |
|
Nicotinic acid
(Niacin B3) |
0.2 | mg | 2.0e-4 | 0.002 | |
| L-Phenylalanine | 0.2 | mg | 2.0e-4 | 0.001 | |
| Biotin | 0.2 | mg | 2.0e-4 | 8.2e-4 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Store in the dark and cold (5°C). | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Edit in Medium builder | |