1647 .
ACETOBACTEROIDES MEDIUM
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Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 0.20 | g | ||
|
|
Glucose | 0.18 | g | ||
|
|
KH2PO4 | 0.14 | g | ||
|
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MgCl2 x 6 H2O | 0.20 | g | ||
|
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CaCl2 x 2 H2O | 0.15 | g | ||
|
|
NH4Cl | 0.54 | ml | ||
|
|
L-Cysteine HCl x H2O | 0.30 | g | ||
|
|
NaHCO3 | 2.50 | g | ||
|
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Na2S x 9 H2O | 0.30 | g | ||
|
|
Resazurin | 1.00 | mg | ||
|
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Vitamin solution | 1.00 | ml | ||
|
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Trace element solution | 1.00 | ml | ||
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Se/W solution | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 The vitamin solution, trace element solution, Se/W solution, cysteine, glucose, Na2S and NaHCO3 are added after the medium has been autoclaved. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Biotin | 2.0 | mg | ||
|
|
Folic acid | 2.0 | mg | ||
|
|
Pyridoxine hydrochloride | 10.0 | mg | ||
|
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Thiamine-HCl x 2 H2O | 5.0 | mg | ||
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Riboflavin | 5.0 | mg | ||
|
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Nicotinic acid | 5.0 | mg | ||
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D-Calcium pantothenate | 5.0 | mg | ||
|
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Vitamin B12 | 0.1 | mg | ||
|
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p-Aminobenzoic acid | 5.0 | mg | ||
|
|
Lipoic acid | 5.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Note: for preparation see Medium 141 | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid
(NTA) |
12.80 | g | ||
|
|
FeCl2 x 4 H2O | 1.00 | g | ||
|
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MnCl2 x 4 H2O | 0.10 | g | ||
|
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CoCl2 x 6 H2O | 0.03 | g | ||
|
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CaCl2 x 2 H2O | 0.10 | g | ||
|
|
ZnCl2 | 0.10 | g | ||
|
|
CuCl2 | 0.02 | g | ||
|
|
H3BO3 | 0.01 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
NiCl2 x 6 H2O | 0.10 | g | ||
|
|
NaCl | 1.00 | g | ||
|
|
Na2SeO3 x 5 H2O | 0.03 | g | ||
|
|
Na2WO4 x 2 H2O | 0.04 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml. | |||||