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DSMZ 1649 . ASW-HD (1:10 diluted)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 0.50 g 0.5 -
Glucose 0.10 g 0.1 0.555
Yeast extract 0.25 g 0.25 -
Agar , for solid medium 15.00 g 15 44.599
Sea water
(Biomaris)
1000.00 ml - -
1 Add to 1000 ml of medium after autoclaving:
Trace element solution SL-10 1.00 ml - -
Vitamin solution 1.00 ml - -
2 Adjust to pH 7.0
Solution occurs in 262 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Medium type: Complex medium
Final pH: 7.0

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Casein peptone 0.5 -
Yeast extract 0.25 -
Glucose 0.1 0.555
HCl 0.003 0.069
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