1660 .
modified BAP+ (PSA) MEDIUM FOR FRANKIA
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Equipment needed:
Autoclave
Final pH:
6.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.950 | g | ||
|
|
K2HPO4 | 0.600 | g | ||
|
|
NH4Cl | 0.270 | g | ||
|
|
Na-propionate | 0.480 | g | ||
|
|
Sodium succinate dibasic | 0.480 | g | ||
|
|
Pyruvic acid sodium salt | 0.480 | g | ||
|
|
Sodium acetate | 0.480 | g | ||
|
|
MgSO4 x 7 H2O | 0.025 | g | ||
|
|
CaCl2 x 2 H2O | 0.010 | g | ||
|
|
Vitamine solution | 1.000 | ml | ||
|
|
Chelated iron solution | 1.000 | ml | ||
|
|
Trace element solution | 0.100 | ml | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 The pH of the final solution is adjusted to 6.3 | |||||
| 2 Note: Please autoclave the chelated iron solution before you add to the medium, so that solves everything!!!! | |||||
Vitamine solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Thiamine HCl | 0.01 | g |
|
|
Nicotinic acid | 0.05 | g |
|
|
Pyridoxine hydrochloride | 0.05 | g |
|
|
Distilled water | 100.00 | ml |
Chelated iron solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Citric acid | 1 | g |
|
|
Eisencitrat | 1 | g |
|
|
Distilled water | 100 | ml |
Trace element solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
CoSO4 x 7 H2O | 1.000 | mg |
|
|
CuSO4 x 5 H2O | 0.080 | g |
|
|
H3BO3 | 2.860 | g |
|
|
MnCl2 x 4 H2O | 1.810 | g |
|
|
Na2MoO4 x 2 H2O | 0.025 | g |
|
|
ZnSO4 x 7 H2O | 0.220 | g |
|
|
Distilled water | 100.000 | ml |