1664 .
Sphaerotilus natans subspec. sulfidivorans medium
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Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)Cl | 0.30 | g | ||
|
|
KH2PO4 | 9.00 | mg | ||
|
|
K2HPO4 | 22.00 | mg | ||
|
|
Na2HPO4 x 7 H2O | 35.00 | mg | ||
|
|
MgSO4 x 7 H2O | 23.00 | mg | ||
|
|
CaCl2 | 28.00 | mg | ||
|
|
FeCl3 x 6 H2O | 0.25 | mg | ||
|
|
Na-lactate | 200.00 | mg | ||
|
|
Peptone | 500.00 | mg | ||
|
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Na2S2SO3 | 1.00 | g | ||
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Trace element solution | 25.00 | μl | ||
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Distilled water | 1000.00 | ml | ||
|
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Standard vitamin solution | 25.00 | μl | ||
| 1 Dissolve ingredients (except vitamins) in distilled water. | |||||
| 2 After sterilization add the 25 µl vitamin solution. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
EDTA | 0.20 | g |
|
|
FeSO4 x 7 H2O | 0.70 | g |
|
|
ZnSO4 x 7 H2O | 0.01 | g |
|
|
MnSO4 x 4 H2O | 2.00 | mg |
|
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CuSO4 x 5 H2O | 5.00 | mg |
|
|
H3BO3 | 0.01 | g |
|
|
Co(NO3)2 | 1.00 | g |
|
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Na2MoO4 x 2 H2O | 1.00 | g |
|
|
Distilled water | 1000.00 | ml |
Standard vitamin solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Riboflavin | 10.0 | mg |
|
|
Thiamine-HCl x 2 H2O | 50.0 | mg |
|
|
Nicotinic acid | 50.0 | mg |
|
|
Pyridoxine hydrochloride | 50.0 | mg |
|
|
Calcium pantothenate | 50.0 | mg |
|
|
Biotin | 0.1 | mg |
|
|
Folic acid | 0.2 | mg |
|
|
Vitamin B12 | 1.0 | mg |
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|
Distilled water | 100.0 | ml |