166 .
HYPHOMICROBIUM STRAIN X MEDIUM
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Equipment needed:
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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(NH4)2SO4 | 1.00 | g | ||
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MgSO4 x 7 H2O | 0.20 | g | ||
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NaH2PO4 x H2O | 0.50 | g | ||
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K2HPO4 | 1.55 | g | ||
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Trace element solution (Vishniac & Santer, 1957) | 0.20 | ml | ||
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Methylamine hydrochloride | 3.40 | g | ||
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Agar | 15.00 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.2. For liquid medium use only 0.7 g of methylamine hydrochloride per liter. | |||||
Trace element solution (Vishniac & Santer, 1957) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 50.00 | g | ||
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ZnSO4 x 7 H2O | 22.00 | g | ||
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CaCl2 x 2 H2O | 5.54 | g | ||
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MnCl2 x 4 H2O | 5.06 | g | ||
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FeSO4 x 7 H2O | 5.00 | g | ||
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(NH4)6Mo7O24 x 4 H2O | 1.10 | g | ||
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CuSO4 x 5 H2O | 1.57 | g | ||
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CoCl2 x 6 H2O | 1.61 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH. | |||||