1679 .
Cyanobacteria Medium 2xASN3
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Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl
(250 g/l stock solution) |
200.0 | g/l | ||
|
|
MgCl2 x 6 H2O
(200 g/l stock solution) |
20.0 | g/l | ||
|
|
KCl
(50 g/l stock solution) |
20.0 | g/l | ||
|
|
Mg(SO4) x 7 H2O
(350 g/l stock solution) |
20.0 | g/l | ||
|
|
CaCl2 x 2 H2O
(50 g/l stock solution) |
20.0 | g/l | ||
|
|
Na3-citrate x 2 H2O
(0.6 g/l stock solution) |
10.0 | ml/l | ||
|
|
Na2-EDTA x 2 H2O
(0.1 g/l stock solution) |
10.0 | ml/l | ||
|
|
T-Trace Metal Mix | 1.0 | ml | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust to 1000 mL with H2O dist. and autoclave. | |||||
| 2 After cooling, add the following filter sterilized components: | |||||
|
|
NaNO3
(300 g/l stock solution) |
2.5 | ml/l | ||
|
|
K2HPO4 x 3 H2O
(8.0 g/l stock solution) |
2.5 | ml/l | ||
|
|
Na2CO3
(20 g/l stock solution) |
1.0 | ml/l | ||
|
|
Fe(NH4)citrate
(6.0 g/l stock solution) |
0.5 | ml/l | ||
|
|
Vitamin B12
(0.02 g/l stock solution) |
1.0 | ml/l | ||
T-Trace Metal Mix #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
H3BO3 | 2.90 | g/l |
|
|
MnCl2 x 4 H2O | 1.81 | g/l |
|
|
ZnSO4 x 7 H2O | 0.22 | g/l |
|
|
Na2MoO4 x 2 H2O | 0.39 | g/l |
|
|
CuSO4 x 5 H2O
(0.08 g/l stock solution) |
10.00 | ml/l |
|
|
Co(NO3)2 x 6 H2O
(0.05 g/l stock solution) |
10.00 | ml/l |