This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution A | 994.0 | ml | - | - | |
Solution B | 0.1 | ml | - | - | |
Solution C | 0.1 | ml | - | - | |
1 Sparge solution A with 100% N2 gas for 30 - 45 min to make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B and C are autoclaved separately under 100% N2 gas. Adjust pH of complete medium to 7.2 - 7.4, if necessary. | |||||
2 Use 1.10 g/l Na-pyruvate as substrate. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
KH2PO4 | 0.020 | g | 0.02 | 0.148 |
NH4Cl | 0.025 | g | 0.025 | 0.47 |
NaCl | 0.100 | g | 0.101 | 1.721 |
MgCl2 x 6 H2O | 0.040 | g | 0.04 | 0.198 |
KCl | 0.050 | g | 0.05 | 0.675 |
CaCl2 x 2 H2O | 0.015 | g | 0.015 | 0.103 |
NaNO3 | 0.850 | g | 0.855 | 10.061 |
Yeast extract | 0.100 | g | 0.101 | - |
Sodium resazurin
(0.1% w/v) |
0.050 | ml | 5.0e-5 | - |
Distilled water | 994.000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Selenite-tungstate solution | 1 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.5 | g | 0.5 | 12.501 |
Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.2 - 7.4 |
Equipment needed: | Autoclave, Filter, Hungate tubes |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaNO3 | 0.855 | 10.061 |
NaCl | 0.101 | 1.721 |
Yeast extract | 0.101 | - |
KCl | 0.05 | 0.675 |
MgCl2 x 6 H2O | 0.04 | 0.198 |
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