Name: Acidobacteriaceae Medium (Modified VSB Medium)
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1694 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.20	g	0.2	1.47
NH₄Cl	0.25	g	0.25	4.674
KCl	0.50	g	0.5	6.707
CaCl₂ x 2 H₂O	0.15	g	0.15	1.02
NaCl	1.00	g	1	17.112
MgCl₂ x 6 H₂O	0.62	g	0.62	3.05
Yeast extract	0.15	g	0.15	-
Proteose peptone	0.15	g	0.15	-
Casamino acids	0.15	g	0.15	-
Glucose	0.45	g	0.45	2.498
HEPES (1 M, pH 7.0)	10.00	ml	2.383	10
Na₂SO₄ (1.25 M)	16.00	ml	2.841	20
Distilled water	1000.00	ml	-	-
1 adjust pH to 5.0
2 Add to 1000 ml of medium after autoclaving:
Trace element solution SL-10	1.00	ml	-	-
Vitamin Mix solution	1.00	ml	-	-
Vitamin B₁₂ (50 mg/l)	0.50	ml	-	-

Trace element solution SL-10 #

1000

Solution occurs in 265 other media (first defined in Medium 320).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	10.0	ml	2.5	68.568
FeCl₂ x 4 H₂O	1.5	g	1.5	7.545
ZnCl₂	70.0	mg	0.07	0.514
MnCl₂ x 4 H₂O	100.0	mg	0.1	0.505
H₃BO₃	6.0	mg	0.006	0.097
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	990.0	ml	-	-
1 First dissolve FeCl₂ in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

100

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na-Phosphate-Buffer (10 mM pH 7.1)	100	ml	-	1
p-Aminobenzoic acid	4	mg	0.04	0.292
D-(+)-biotin	1	mg	0.01	0.041
Nicotinic acid	10	mg	0.1	0.812
D-Calcium pantothenate	5	mg	0.05	-
Pyridoxine hydrochloride	15	mg	0.15	0.729

All strains for this medium:

No strains found

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