1696
1696 .
MEDIUM for Z-1701, Z-1702
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1696 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.0 | g | 20 | 342.231 | |
| Na2CO3 | 50.0 | g | 50 | 471.752 | |
| NaHCO3 | 60.0 | g | 60 | 714.226 | |
| KH2PO4 | 0.2 | g | 0.2 | 1.47 | |
| MgCl2 x 6 H2O | 0.1 | g | 0.1 | 0.492 | |
| Trace element solution | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Final pH 9.0 - 9.5 | |||||
| 2 Prepare and boil the medium in the absence NaHCO3, Na2CO3.Cool under a stream of nitrogen and add the remaining components. Dispense into tubes or bottle fitted with rubber stoppers and autoclave. | |||||
Solution occurs in 1
other media (first defined in Medium 1374).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Fe(NH4)2(SO4)2 x 6 H2O | 784.0 | mg | 0.784 | - |
| ZnSO4 x 7 H2O | 143.6 | mg | 0.144 | 0.499 |
| MnCl2 x 4 H2O | 99.0 | mg | 0.099 | 0.5 |
| Na2SeO4 | 94.6 | mg | 0.095 | 0.501 |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 |
| Na2WO4 x 2 H2O | 33.0 | mg | 0.033 | 0.1 |
| CuCl2 x 2 H2O | 1.8 | mg | 0.002 | 0.011 |
| CoCl2 x 6 H2O | 238.0 | mg | 0.238 | 1.833 |
| (NH4)2Ni(SO4)2 x 6 H2O | 395.0 | mg | 0.395 | 1 |
| Na2MoO4 x 2 H2O | 24.0 | mg | 0.024 | 0.099 |
|
HCl
(concentrated) |
10.0 | ml | - | - |
| Distilled water | 990.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Defined medium |
| Final pH: | 9.0 - 9.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 60 | 714.226 |
| Na2CO3 | 50 | 471.752 |
| NaCl | 20 | 342.231 |
| KH2PO4 | 0.2 | 1.47 |
| MgCl2 x 6 H2O | 0.1 | 0.492 |
| Show more | ||
