1708
1708 .
THERMOANAEROMYCES MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1708 #
ml
Equipment needed: Autoclave
Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.35 | g | 0.35 | 2.009 | |
| KH2PO4 | 0.23 | g | 0.23 | 1.69 | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| NaCl | 2.25 | g | 2.25 | 38.501 | |
| FeSO4 x 7 H2O | 2.00 | mg | 0.002 | 0.007 | |
| Yeast extract | 2.00 | g | 2 | - | |
| Lactose | 1.00 | g | 1 | 2.921 | |
| Na-acetate | 0.50 | g | 0.5 | 6.095 | |
| NaHCO3 | 0.50 | g | 0.5 | 5.952 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
1.00 | ml | 1.0e-3 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Add after autoclaving 0.25/50 ml of sterile Na2S x 9 H2O (stock solution 12 g/l) | |||||
| 2 Dissolve ingredients (except bicarbonate, trace elements, resazurin, cysteine and sulfide) and sparge medium with N2 for 30 - 45 min to make it anoxic. Then add and dissolve L-cysteine and bicarbonate, adjust pH to 6.8 and dispense medium under N2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. | |||||
| 3 The reducing agent is autoclaved separately under 100% N2 gas atmosphere as concentrated solutions in tightly closed tubes. Trace elements are prepared under 100% N2 gas atmosphere and sterilized by filtration. Appropriate volumes of the stock solutions are injected into the sterile medium with hypodermic syringes. Adjust pH of the complete medium to 6.8 - 7.0, if necessary. | |||||
Solution occurs in 265
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 - 7.0 |
| Equipment needed: | Autoclave, Filter, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 2.25 | 38.501 |
| Yeast extract | 2 | - |
| Lactose | 1 | 2.921 |
| NH4Cl | 0.5 | 9.348 |
| Na-acetate | 0.5 | 6.095 |
| Show more | ||
