172a .
CY S2 MEDIUM
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Equipment needed:
Autoclave
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NH4Cl | 1.00 | g | ||
|
|
KH2PO4 | 1.00 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
CaCl2 x 2 H2O | 0.06 | g | ||
|
|
NaCl | 30.00 | g | ||
|
|
FeCl3 x 6 H2O | 1.25 | mg | ||
|
|
Yeast extract | 1.00 | g | ||
|
|
Agar | 1.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.0 and autoclave. | |||||
| 2 After autoclaving, add from separate sterile solutions per liter medium: | |||||
|
|
Glucose
(10% w/v) |
10.00 | ml | ||
|
|
NaHCO3
(5% w/v) |
10.00 | ml | ||
|
|
Na2S x 9 H2O
(3% w/v) |
3.30 | ml | ||
| 3 Aseptically fill sterile 10-ml screwcap tubes almost completely with the medium. Make stab inoculation. | |||||