173
173 .
OCEANISPIROCHAETA LITORALIS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 173 #
ml
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| D-Glucose | 2.0 | g | 2 | 11.101 | |
|
Trypticase peptone
(BD BBL) |
1.0 | g | 1 | - | |
|
Yeast extract
(OXOID) |
1.0 | g | 1 | - | |
|
Tris-HCl buffer
(1 M, pH 7.5) |
50.0 | ml | - | 50 | |
| Sea water | 750.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Distilled water | 200.0 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine) and sparge medium for 30 - 45 min with 100% N2 gas to make it anoxic. Add cysteine and adjust pH to 7.2 - 7.4, then distribute medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Final pH of the medium should be about 7.2. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| D-Glucose | 2 | 11.101 |
| Yeast extract | 1 | - |
| Trypticase peptone | 1 | - |
| L-Cysteine HCl x H2O | 0.5 | 2.847 |
| Sodium resazurin | 5.0e-4 | - |
| Show more | ||
