DSMZ 1743 . GHIORSEA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter Gassing station Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.50 g 27.5 470.568
MgCl2 x 6 H2O 5.38 g 5.38 26.463
MgSO4 x 7 H2O 6.78 g 6.78 27.508
KCl 0.72 g 0.72 9.658
NH4Cl 0.50 g 0.5 9.348
CaCl2 x 2 H2O 1.40 g 1.4 9.523
KH2PO4 0.05 g 0.05 0.367
Trace element solution 10.00 ml - -
NaHCO3 1.70 g 1.7 20.236
Wolin's vitamin solution 10.00 ml - -
Distilled water 980.00 ml - -
1 Dissolve ingredients (except vitamins), then sparge medium with 80% N2 and 20% CO2 gas mixture to make it anoxic and until a pH of 6.5 is reached. Dispense medium under same gas atmosphere into anoxic serum vials to 20% of their volume and autoclave. After autoclaving add vitamins from a sterile stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration. Prior to inoculation add 5% (v/v) sterile H2 and 5% (v/v) sterile air to the headspace by using syringes. Check pH of the complete medium and adjust pH to 6.5, if necessary. Incubate without shaking.
2 Note: After growth has started cell density of the culture can be increased by replenishing H2 and air every two days.
Solution occurs in 2 other media (first defined in Medium 705).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 0.50 g 0.5 1.487
CoCl2 x 6 H2O 0.15 g 0.15 1.155
MnCl2 x 4 H2O 0.10 g 0.1 0.505
FeSO4 x 7 H2O 0.10 g 0.1 0.36
ZnCl2 0.10 g 0.1 0.734
AlCl3 x 6 H2O 40.00 mg 0.04 0.166
Na2WO4 x 2 H2O 40.00 mg 0.04 0.121
Na2SeO3 x 5 H2O 30.00 mg 0.03 0.114
NiCl2 x 6 H2O 20.00 mg 0.02 0.154
CuCl2 x 2 H2O 20.00 mg 0.02 0.117
H3BO3 10.00 mg 0.01 0.162
Na2MoO4 x 2 H2O 10.00 mg 0.01 0.041
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7 using 2 N NaOH, then dissolve remaining compounds.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 12.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Gassing station, Serum bottle
Edit in Medium builder

Gas composition

  • 73 % N2
  • 18 % CO2
  • 5 % Air
  • 5 % H2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 27.5 470.568
MgSO4 x 7 H2O 6.78 27.508
MgCl2 x 6 H2O 5.38 26.463
NaHCO3 1.7 20.236
CaCl2 x 2 H2O 1.4 9.523
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain