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DSMZ

DSMZ 1743 . GHIORSEA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1743 #

ml
Equipment needed: Autoclave Filter Gassing station Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.50 g 27.201 465.448
MgCl2 x 6 H2O 5.38 g 5.321 26.175
MgSO4 x 7 H2O 6.78 g 6.706 27.209
KCl 0.72 g 0.712 9.553
NH4Cl 0.50 g 0.495 9.246
CaCl2 x 2 H2O 1.40 g 1.385 9.419
KH2PO4 0.05 g 0.049 0.363
Trace element solution 10.00 ml - -
NaHCO3 1.70 g 1.682 20.016
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except vitamins), then sparge medium with 80% N2 and 20% CO2 gas mixture to make it anoxic and until a pH of 6.5 is reached. Dispense medium under same gas atmosphere into anoxic serum vials to 20% of their volume and autoclave. After autoclaving add vitamins from a sterile stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration. Prior to inoculation add 5% (v/v) sterile H2 and 5% (v/v) sterile air to the headspace by using syringes. Check pH of the complete medium and adjust pH to 6.5, if necessary. Incubate without shaking.
2 Note: After growth has started cell density of the culture can be increased by replenishing H2 and air every two days.

Trace element solution #

1000
Solution occurs in 2 other media (first defined in Medium 705).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 0.50 g 0.5 1.487
CoCl2 x 6 H2O 0.15 g 0.15 1.155
MnCl2 x 4 H2O 0.10 g 0.1 0.505
FeSO4 x 7 H2O 0.10 g 0.1 0.36
ZnCl2 0.10 g 0.1 0.734
AlCl3 x 6 H2O 40.00 mg 0.04 0.166
Na2WO4 x 2 H2O 40.00 mg 0.04 0.121
Na2SeO3 x 5 H2O 30.00 mg 0.03 0.114
NiCl2 x 6 H2O 20.00 mg 0.02 0.154
CuCl2 x 2 H2O 20.00 mg 0.02 0.117
H3BO3 10.00 mg 0.01 0.162
Na2MoO4 x 2 H2O 10.00 mg 0.01 0.041
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7 using 2 N NaOH, then dissolve remaining compounds.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 12.04.23
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Gassing station, Serum bottle
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Gas composition

  • 76 % N2
  • 18 % CO2
  • 5 % H2
  • 1 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 27.201 465.448
MgSO4 x 7 H2O 6.706 27.209
MgCl2 x 6 H2O 5.321 26.175
NaHCO3 1.682 20.016
CaCl2 x 2 H2O 1.385 9.419
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain