1790 .
Mucispirillum Spezial
What is this page?
Equipment needed:
Autoclave
Filter
Final pH:
7.0
Description:
Special medium for Mucispirillum schaedleri.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Brain heart infusion | 9.260 | g | ||
|
|
Trypticase soy broth | 7.500 | g | ||
|
|
Yeast extract | 2.500 | g | ||
|
|
NaNO3 | 0.426 | g | ||
|
|
K2HPO4 | 1.250 | g | ||
| 1 Adjust pH to 7.0. | |||||
| 2 Bring to boil, cool down under N2CO2. | |||||
|
|
Cysteine HCl | 0.250 | g | ||
| 3 Fill under N2CO2 in hungate tubes (10 ml) | |||||
| 4 Autoclave at 121 °C for 15 min. | |||||
|
|
Haemin solution | 0.050 | ml | ||
|
|
Vitamin K3 solution | 0.050 | ml | ||
|
|
Fetal bovine serum | 0.500 | ml | ||
| 5 pressurize with 1 bar H2 | |||||
Haemin solution #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Haemin | 50 | mg | ||
|
|
NaOH
(1 N) |
1 | ml | ||
|
|
Distilled water | 100 | ml | ||
| 1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. | |||||
Vitamin K3 solution #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Vitamin K3 | 5 | mg | ||
|
|
Ethanol
(95%) |
1 | ml | ||
|
|
Water | 100 | ml | ||
| 1 Dissolve 5 mg vitamin K3 in 1 ml 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle. | |||||