182 .
SACCHAROLOBUS SOLFATARICUS MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
4.0 - 4.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract
(OXOID) |
1.00 | g | ||
|
|
Casamino acids
(BD BBL) |
1.00 | g | ||
|
|
KH2PO4 | 3.10 | g | ||
|
|
(NH4)2SO4 | 2.50 | g | ||
|
|
MgSO4 x 7 H2O | 0.20 | g | ||
|
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
|
Allen's trace element solution | 10.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve all compounds except yeast extract and Casamino acids, adjust pH to 4.0 - 4.2 with 10 N H2SO4 at room temperature and autoclave. Prior to inoculation add yeast extract and Casamino acids from stock solutions sterilized by filtration. | |||||
Allen's trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MnCl2 x 4 H2O | 180 | mg | ||
|
|
Na2B4O7 x 10 H2O | 450 | mg | ||
|
|
ZnSO4 x 7 H2O | 22 | mg | ||
|
|
CuCl2 x 2 H2O | 5 | mg | ||
|
|
Na2MoO4 x 2 H2O | 3 | mg | ||
|
|
VOSO4 x 2 H2O | 3 | mg | ||
|
|
CoSO4 x 7 H2O | 1 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH of final solution to 2 with 1 N HCl. | |||||