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DSMZ

DSMZ 211 . THERMOANAEROBACTERIUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Thermoanaerobacterium thermosulfurigenes DSM 2229

This strain uses the standard medium without modifications.

Growth conditions: anaerobic 60 °C

Main sol. 211 #

ml
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.3 g 0.295 2.168
Na2HPO4 x 12 H2O 5.3 g 5.211 36.711
NH4Cl 1.0 g 0.983 18.383
MgCl2 x 6 H2O 0.2 g 0.197 0.967
Trace element solution 10.0 ml - -
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) 		1.5 ml 0.001 0.005
Yeast extract 1.0 g 0.983 -
Sodium resazurin
(0.1% w/v) 		0.5 ml 4.9e-4 -
D-Glucose 5.0 g 4.916 27.289
Wolin's vitamin solution 5.0 ml - -
Na2S x 9 H2O 0.5 g 0.492 2.047
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except glucose, vitamins and sulfide), adjust pH to 6.0, sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into Hungate-type tubes or serum vials and autoclave. After sterilization add glucose and sulfide from anoxic stock solutions autoclaved under 100% N2 gas and vitamins from a filter-sterilized anoxic stock solution prepared under 100% N2. Adjust pH of complete medium to 6.0 - 6.5, if necessary.

Trace element solution #

1000
Solution occurs in 8 other media (first defined in Medium 144).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		12.80 g 12.8 66.967
FeCl2 x 4 H2O 0.20 g 0.2 1.006
MnCl2 x 4 H2O 0.10 g 0.1 0.505
CoCl2 x 6 H2O 0.17 g 0.17 1.309
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnCl2 0.10 g 0.1 0.734
CuCl2 0.02 g 0.02 0.149
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
NaCl 1.00 g 1 17.112
Na2SeO3 x 5 H2O 0.03 g 0.03 0.114
Distilled water 1000.00 ml - -
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.

Wolin's vitamin solution #

1000
Solution occurs in 53 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.0 - 6.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 6
Strains with modifications: 3
Thermoanaerobacterium thermosulfurigenes DSM 2229

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 5.211 36.711
D-Glucose 4.916 27.289
NH4Cl 0.983 18.383
Yeast extract 0.983 -
Na2S x 9 H2O 0.492 2.047
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Cultivation metadata from 6 strains BacDive

pH 2 - 4 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 6 strains
oxygen anaerobe 6 strains
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