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141. METHANOGENIUM MEDIUM (H2/CO2)

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.34 g 0.333 4.462
2 4.00 g 3.914 19.252
3 3.45 g 3.376 13.696
4 0.25 g 0.245 4.573
5 0.14 g 0.137 0.932
6 0.14 g 0.137 0.786
7 18.00 g 17.613 301.365
8 Trace element solution 10.00 ml - -
9
(0.1% w/v)
2.00 ml 0.002 0.005
10 1.00 g 0.978 11.928
11
(OXOID)
2.00 g 1.957 -
13
(0.1% w/v)
0.50 ml 4.9e-4 0.002
14 5.00 g 4.892 58.238
15 Vitamin solution 10.00 ml - -
16 0.50 g 0.489 2.786
17 0.50 g 0.489 2.037
18 1000.00 ml 976.719 54216.1
19
(1 N)
optional
x - -
Instructions:
  1. Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate and adjust pH to 7.0, then dispense medium under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of complete medium to 6.8 - 7.0, if necessary. For incubation use sterile 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure.
  2. Note: If the medium is being used without overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary.
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
17 , required for pH adjustment x - -
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 31
Strains with modifications: 31

Gas composition

  • 20 % CO2
  • 80 % H2

Light

36 strains need light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.253 55300.4
NaCl 17.622 301.532
NaHCO3 4.892 58.238
MgCl2 x 6 H2O 3.914 19.252
MgSO4 x 7 H2O 3.405 13.815
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Cultivation metadata from 31 strains BacDive

halophily not 5 strains
halophily moderate 5 strains
halophily slightly 4 strains
pH 6 - 8 6 strains
pH 4 - 6 5 strains
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