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Modified Wolin's mineral solution
Internal Solution-ID: S241
This solution is used in 169 media and was first defined in medium 141.
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
| Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||
Media with this solution
| 135b | ACETOBACTERIUM DEHALOGENANS MEDIUM |
| 135a | ACETOBACTERIUM MEDIUM (MARINE) |
| 135 | ACETOBACTERIUM MEDIUM |
| 135c | MOORELLA THERMOACETICA MEDIUM |
| 141b | METHANOCULLEUS SP. MEDIUM |
| 141 | METHANOGENIUM MEDIUM (H2/CO2) |
| 141a | METHANOCALDOCOCCUS LAUENSIS MEDIUM |
| 141c | METHANOCOCCOIDES MEDIUM |
| 161 | METHANOFOLLIS MEDIUM |
| 165 | ACETIVIBRIO CELLULOLYTICUS MEDIUM |
| 171 | THERMOANAEROBACTER KIVUI MEDIUM |
| 203 | METHANOTHERMUS MEDIUM |
| 233 | METHANOLOBUS TINDARIUS MEDIUM |
| 274 | METHANOLACINIA MEDIUM |
| 280 | METHANOCOCCOIDES MEDIUM (N2/CO2) |
| 282b | DESULFUROBACTERIUM HR11 MEDIUM |
| 282a | METHANOCALDOCOCCUS VILLOSUS MEDIUM |
| 282 | METHANOCALDOCOCCUS MEDIUM |
| 283 | PYRODICTIUM MEDIUM |
| 284 | KOSMOTOGA MEDIUM |
| 287 | METHANOCULLEUS CHIKUGOENSIS MEDIUM |
| 288 | METHANOCOCCUS MARIPALUDIS MEDIUM |
| 316 | MOORELLA THERMOACETICA (TYE-CO) MEDIUM |
| 317 | METHANOSARCINA THERMOPHILA MEDIUM |
| 319 | SPOROHALOBACTER LORTETII MEDIUM |
| 321 | METHANOCORPUSCULUM AGGREGANS MEDIUM |
| 322 | METHANOSPHAERA MEDIUM |
| 342 | METHANOBACTERIUM ALCALIPHILUM MEDIUM |
| 343 | THERMOTOGA NEAPOLITANA MEDIUM |
| 343a | THERMOSIPHO MELANESIENSIS MEDIUM |
| 373 | METHANOGENIUM CV MEDIUM |
| 377a | PALAEOCOCCUS MEDIUM |
| 377 | PYROCOCCUS MEDIUM |
| 377b | PYROCOCCUS ST04 MEDIUM |
| 380a | TELMATOSPIRILLUM (PEAT) MEDIUM |
| 380 | MAGNETOSPIRILLUM MEDIUM |
| 391 | METHANOCOCCUS VOLTAE MEDIUM |
| 396 | METHANOSALSUM ZHILINAE MEDIUM |
| 399 | ARCHAEOGLOBUS MEDIUM |
| 404 | METHANOSPHAERA CUNICULI MEDIUM |
| 408 | PSYCHRILYOBACTER SP. MEDIUM |
| 412 | ACETOMICROBIUM FAECALIS MEDIUM |
| 477 | ACIDIHALOBACTER PROSPERUS MEDIUM |
| 479a | METHANOHALOPHILUS PROFUNDI MEDIUM |
| 479 | METHANOHALOPHILUS MEDIUM |
| 481 | COPROTHERMOBACTER PROTEOLYTICUS MEDIUM |
| 483 | DEFLUVIITALEA MEDIUM |
| 490 | METHANOLOBUS OREGONENSIS MEDIUM |
| 491 | HYPERTHERMUS BUTYLICUS MEDIUM |
| 493 | THIOMONAS (HÖ5) MEDIUM |
| 494 | ACETOHALOBIUM MEDIUM |
| 498 | PSEUDOTHERMOTOGA MEDIUM |
| 503b | FWM MEDIUM (PERCHLORATE) |
| 506 | METHANOBACTERIUM ESPANOLAE MEDIUM |
| 511 | METHANOPYRUS MEDIUM |
| 519 | ARCHAEOGLOBUS PROFUNDUS MEDIUM |
| 525 | METHANOHALOPHILUS (MG) MEDIUM |
| 525a | METHANOHALOPHILUS (MG+NaCl) MEDIUM |
| 540 | ACETITOMACULUM MEDIUM |
| 558a | CLOSTRIDIUM (RB-9) MEDIUM |
| 558 | CLOSTRIDIUM (RB-1) MEDIUM |
| 561 | SULFURIFERULA MEDIUM |
| 579 | GEOBACTER MEDIUM |
| 611 | PYROBACULUM (BS) MEDIUM |
| 613 | THERMOTOGA TF(C) MEDIUM |
| 614 | ACETOBACTERIUM FIMETARIUM MEDIUM |
| 635 | GRACILINEMA MEDIUM |
| 643 | SEDIMENTIBACTER MEDIUM |
| 664a | PSEUDOTHERMOTOGA LETTINGAE MEDIUM |
| 664 | PSEUDOTHERMOTOGA ELFII MEDIUM |
| 671 | THERMOANAEROBACTER (BA) MEDIUM |
| 678 | COPROTHERMOBACTER (CP) MEDIUM |
| 685 | ANAEROBRANCA MEDIUM |
| 688 | PSEUDOTHERMOTOGA SUBTERRANEA MEDIUM |
| 690 | DMA MEDIUM |
| 692 | METHANOSALSUM WeN5 MEDIUM |
| 713 | METHANOHALOPHILUS EUHALOBIUS MEDIUM |
| 716 | AMMONIFEX DEGENSII MEDIUM |
| 717 | DESULFITOBACTERIUM (PCE) MEDIUM |
| 718a | PETROTOGA HALOPHILA MEDIUM |
| 718 | PETROTOGA MEDIUM |
| 730 | FERROGLOBUS PLACIDUS MEDIUM |
| 731 | THERMOSYNTROPHA MEDIUM |
| 733 | SULFITOBACTER PONTIACUS MEDIUM |
| 738 | BPHD Medium |
| 740 | FERVIDOBACTERIUM TF(A) MEDIUM |
| 742 | DESULFONATRONOVIBRIO MEDIUM |
| 749 | THERMODESULFOVIBRIO YELLOWSTONII MEDIUM |
| 786 | DETHIOSULFOVIBRIO PEPTIDOVORANS MEDIUM |
| 794 | PSEUDOTHERMOTOGA HYPOGEA MEDIUM |
| 795 | STETTERIA MEDIUM |
| 795a | LEBETIMONAS MEDIUM |
| 814 | METHANOBACTERIUM SUBTERRANEUM MEDIUM |
| 819 | SEDIMINISPIROCHAETA SMARAGDINAE MEDIUM |
| 825 | METHANOBACTERIUM II MEDIUM |
| 825a | METHANOBACTERIUM II MEDIUM (N2/CO2) |
| 826 | GEOBACTER SULFURREDUCENS MEDIUM |
| 829c | DESULFUROBACTERIUM CRINIFEX MEDIUM |
| 829d | DESULFUROBACTERIUM INDICUM MEDIUM |
| 837 | DESULFUROMONAS PALMITATIS MEDIUM |
| 853a | FUSIBACTER TUNISIENSIS MEDIUM |
| 853 | FUSIBACTER MEDIUM |
| 853b | FUSIBACTER BIZERTENSIS MEDIUM |
| 854 | HIPPEA MEDIUM |
| 870 | SYNTROPHOTHERMUS MEDIUM |
| 879 | CLOSTRIDIUM LJUNGDAHLII MEDIUM |
| 895 | ANAEROBRANCA GOTTSCHALKII MEDIUM |
| 896 | SOLIDESULFOVIBRIO MEDIUM |
| 899 | CALDANAEROBACTER MEDIUM |
| 905 | METHANOCALCULUS HALOTOLERANS MEDIUM |
| 913 | THERMOTOGA PETROPHILA MEDIUM |
| 915 | BLAUTIA MEDIUM |
| 924 | METHANOCALCULUS MB MEDIUM |
| 924b | METHANOSARCINA MB MEDIUM (1 g/l NaCl) |
| 924a | METHANOFOLLIS MB MEDIUM (5 g/l NaCl) |
| 931 | ALCALIPHILIC AMPHIBACILLUS STRAINS MEDIUM |
| 935a | DEFERRIBACTER DESULFURICANS MEDIUM |
| 946a | CALDITHRIX MEDIUM |
| 946 | NAUTILIA MEDIUM |
| 962a | THERMOVENABULUM MEDIUM |
| 962 | THERMINCOLA FERRIACETICA MEDIUM |
| 965 | THERMOANAEROBACTER KREATINOPHILUS MEDIUM |
| 975 | OCEANITHERMUS PROFUNDUS MEDIUM |
| 1011a | THIOBACTER MJ MEDIUM |
| 1011 | SULFURIMONAS MJ MEDIUM |
| 1011d | GALENEA MJ MEDIUM (CO2) |
| 1011c | DESULFOTHERMUS MJ MEDIUM (H2/CO2) |
| 1013 | HALOBACTERIOVORAX MEDIUM |
| 1043 | IGNISPHAERA MEDIUM |
| 1053a | THIOPROFUNDUM LITHOTROPHICUM MEDIUM |
| 1053 | SULFURIMONAS PARALVINELLA MEDIUM |
| 1062 | DESULFITOBACTERIUM (PCE II) MEDIUM |
| 1082 | AMINIPHILUS MEDIUM |
| 1114 | THIOFABA MEDIUM |
| 1163a | OCEANOTOGA MEDIUM |
| 1163 | KOSMOTOGA OLEARIA MEDIUM |
| 1206 | CALDANAEROVIRGA MEDIUM |
| 1232 | THERMOTOGA TF(D) MEDIUM |
| 1234 | ACIDITHIOBACILLUS FERRIVORANS MEDIUM |
| 1236 | SPOROLITUUS MEDIUM |
| 1237 | TEPIDIMICROBIUM MEDIUM |
| 1243 | PARADESULFITOBACTERIUM MEDIUM |
| 1251 | CALDINITRATIRUPTOR MEDIUM |
| 1263 | SPIROCHAETA DISSIPATITROPHA MEDIUM |
| 1267 | FERRIPHASELUS (ES) MEDIUM |
| 1273 | METHANOSPIRILLUM LACUNAE MEDIUM |
| 1278 | ARCHAEOGLOBUS SULFATICALLIDUS MEDIUM |
| 1290 | ALKALIPHILUS MEDIUM |
| 1292 | CALDICOPROBACTER ALGERIENSIS MEDIUM |
| 1484 | Glycerol-Yeast extract-Peptone Medium |
| 1525 | KALLOTENUE MEDIUM |
| 1526e | LIMIHALOGLOBUS MEDIUM |
| 1526 | SALINISPIRA (L21 LS) MEDIUM |
| 1526d | LIMISALSIVIBRIO (L21 LS) MEDIUM |
| 1526c | DESULFOHALOVIBRIO (L21 LS) MEDIUM |
| 1526a | KIRITIMATIELLA (L21 LS) MEDIUM |
| 1527a | SALINIVIRGA (L21 HS) MEDIUM |
| 1527 | SEDIMENTISPHAERA (L21 HS) MEDIUM |
| 1565 | ALKALIPHILUS TRANSVAALENSIS MEDIUM |
| 1570 | THIOGRANUM MEDIUM |
| 1577 | SPOROHALOBACTER SALINUS MEDIUM |
| 1636 | TEPIDIBACILLUS MEDIUM |
| 1662 | ALKALIPHILUS HYDROTHERMALIS MEDIUM |
| 1681 | Mineral salt medium with colloidal chitin |
| 1699 | SULFURIMONAS CRATERIS MEDIUM |
| 1717 | PERLABENTIMONAS MEDIUM |
| 1731 | PONTIELLA MEDIUM |
| 1836 | ANAEROBACCA MEDIUM |
| 1867 | Thauera aminoaromatica medium |