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Modified Wolin's mineral solution

Internal Solution-ID: S241

This solution is used in 171 media and was first defined in medium 141.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
135c MOORELLA THERMOAUTOTROPHICA MEDIUM
135b ACETOBACTERIUM DEHALOGENANS MEDIUM
135a ACETOBACTERIUM MEDIUM (MARINE)
135 ACETOBACTERIUM MEDIUM
141c METHANOCOCCOIDES MEDIUM
141b METHANOCULLEUS SP. MEDIUM
141a METHANOCOCCUS SP. MEDIUM
141 METHANOGENIUM MEDIUM (H2/CO2)
161 METHANOMICROBIUM MOBILE MEDIUM
165 ACETIVIBRIO CELLULOLYTICUS MEDIUM
171 THERMOANAEROBACTER KIVUI MEDIUM
195e DESULFOFERROBACTER MEDIUM
203 METHANOTHERMUS MEDIUM
233 METHANOLOBUS TINDARIUS MEDIUM
274 METHANOLACINIA MEDIUM
280 METHANOCOCCOIDES MEDIUM (N2/CO2)
282 METHANOCALDOCOCCUS MEDIUM
282b DESULFUROBACTERIUM HR11 MEDIUM
282a METHANOCALDOCOCCUS VILLOSUS MEDIUM
283 PYRODICTIUM MEDIUM
284 KOSMOTOGA MEDIUM
287 METHANOGENIUM OLENTANGYI MEDIUM
288 METHANOCOCCUS DELTAE MEDIUM
316 MOORELLA THERMOACETICA (TYE-CO) MEDIUM
317 METHANOSARCINA THERMOPHILA MEDIUM
319 SPOROHALOBACTER LORTETII MEDIUM
321 METHANOCORPUSCULUM AGGREGANS MEDIUM
322 METHANOSPHAERA (MCB-3) MEDIUM
342 METHANOBACTERIUM ALCALIPHILUM MEDIUM
343a Thermosipho melanesiensis MEDIUM
343 THERMOTOGA NEAPOLITANA MEDIUM
373 METHANOGENIUM CV MEDIUM
377b PYROCOCCUS ST04 MEDIUM
377a PALAEOCOCCUS MEDIUM
377 PYROCOCCUS MEDIUM
380a TELMATOSPIRILLUM (PEAT) MEDIUM
380 MAGNETOSPIRILLUM MEDIUM
391 METHANOCOCCUS VOLTAE MEDIUM
396 METHANOSALSUM ZHILINAE MEDIUM
399a ARCHAEOGLOBUS NEPTUNIUS MEDIUM
399 ARCHAEOGLOBUS MEDIUM
404 METHANOSPHAERA CUNICULI MEDIUM
408 PSYCHRILYOBACTER SP. MEDIUM
412 ACETOMICROBIUM FAECALIS MEDIUM
477 ACIDIHALOBACTER PROSPERUS MEDIUM
479a METHANOHALOPHILUS PROFUNDI MEDIUM
479 METHANOHALOPHILUS MEDIUM
481 Coprothermobacter proteolyticus MEDIUM
483 DEFLUVIITALEA MEDIUM
490 METHANOHALOPHILUS OREGONESE MEDIUM
491 HYPERTHERMUS BUTYLICUS MEDIUM
493 THIOMONAS (HÖ5) MEDIUM
494 ACETOHALOBIUM MEDIUM
498 PSEUDOTHERMOTOGA MEDIUM
503b FWM MEDIUM (PERCHLORATE)
506 METHANOBACTERIUM ESPANOLAE MEDIUM
511 METHANOPYRUS MEDIUM
519 ARCHAEOGLOBUS PROFUNDUS MEDIUM
525a METHANOHALOPHILUS (MG+NaCl) MEDIUM
525 METHANOHALOPHILUS (MG) MEDIUM
540 ACETITOMACULUM MEDIUM
558a CLOSTRIDIUM (RB-9) MEDIUM
558 CLOSTRIDIUM (RB-1) MEDIUM
561 SULFURIFERULA MEDIUM
579 GEOBACTER MEDIUM
611 PYROBACULUM (BS) MEDIUM
613 THERMOTOGA TF(C) MEDIUM
614 ACETOBACTERIUM FIMETARIUM MEDIUM
635 TREPONEMA CALDARIUM MEDIUM
643 SEDIMENTIBACTER MEDIUM
664 THERMOTOGA ELFII MEDIUM
664a PSEUDOTHERMOTOGA LETTINGAE MEDIUM
671 THERMOANAEROBACTER (BA) MEDIUM
678 COPROTHERMOBACTER (CP) MEDIUM
685 ANAEROBRANCA MEDIUM
688 THERMOTOGA SUBTERRANEA MEDIUM
690 DMA MEDIUM
692 METHANOSALSUM WeN5 MEDIUM
713 METHANOHALOPHILUS EUHALOBIUS MEDIUM
716 AMMONIFEX DEGENSII MEDIUM
717 DESULFITOBACTERIUM (PCE) MEDIUM
718 PETROTOGA MEDIUM
718a PETROTOGA HALOPHILA MEDIUM
730 FERROGLOBUS PLACIDUS MEDIUM
731 THERMOSYNTROPHA MEDIUM
733 SULFITOBACTER PONTIACUS MEDIUM
738 BPHD Medium
740 FERVIDOBACTERIUM TF(A) MEDIUM
742 DESULFONATRONOVIBRIO MEDIUM
749 THERMODESULFOVIBRIO YELLOWSTONII MEDIUM
786 DETHIOSULFOVIBRIO PEPTIDOVORANS MEDIUM
794 THERMOTOGA HYPOGEA MEDIUM
795a LEBETIMONAS MEDIUM
795 STETTERIA MEDIUM
814 METHANOBACTERIUM SUBTERRANEUM MEDIUM
819 SEDIMINISPIROCHAETA SMARAGDINAE MEDIUM
825a METHANOBACTERIUM II MEDIUM (N2/CO2)
825 METHANOBACTERIUM II MEDIUM
826 GEOBACTER SULFURREDUCENS MEDIUM
829d DESULFUROBACTERIUM INDICUM MEDIUM
829c DESULFUROBACTERIUM CRINIFEX MEDIUM
837 DESULFUROMONAS PALMITATIS MEDIUM
853b FUSIBACTER BIZERTENSIS MEDIUM
853a FUSIBACTER TUNISIENSIS MEDIUM
853 FUSIBACTER PAUCIVORANS MEDIUM
854 HIPPEA MEDIUM
870 SYNTROPHOTHERMUS MEDIUM
879 CLOSTRIDIUM LJUNGDAHLII MEDIUM
895 ANAEROBRANCA GOTTSCHALKII MEDIUM
896 DESULFOVIBRIO MAGNETICUS MEDIUM
899 THERMOANAEROBACTER SUBTERRANEUS MEDIUM
905 METHANOCALCULUS HALOTOLERANS MEDIUM
913 THERMOTOGA PETROPHILA MEDIUM
915 BLAUTIA MEDIUM
924a METHANOFOLLIS MB MEDIUM (5 g/l NaCl)
924 METHANOCALCULUS MB MEDIUM
924b METHANOSARCINA MB MEDIUM (1 g/l NaCl)
931 ALCALIPHILIC AMPHIBACILLUS STRAINS MEDIUM
935a DEFERRIBACTER DESULFURICANS MEDIUM
946 NAUTILIA MEDIUM
946a CALDITHRIX MEDIUM
962a THERMOVENABULUM MEDIUM
962 THERMINCOLA FERRIACETICA MEDIUM
965 THERMOANAEROBACTER KREATINOPHILUS MEDIUM
975 OCEANITHERMUS PROFUNDUS MEDIUM
1011c DESULFOTHERMUS MJ MEDIUM (H2/CO2)
1011a THIOBACTER MJ MEDIUM
1011 SULFURIMONAS MJ MEDIUM
1011d GALENEA MJ MEDIUM (CO2)
1013 HALOBACTERIOVORAX MEDIUM
1043 IGNISPHAERA MEDIUM
1053 SULFURIMONAS PARALVINELLA MEDIUM
1053a THIOPROFUNDUM LITHOTROPHICUM MEDIUM
1062 DESULFITOBACTERIUM (PCE II) MEDIUM
1082 AMINIPHILUS MEDIUM
1114 THIOFABA MEDIUM
1163a OCEANOTOGA MEDIUM
1163 KOSMOTOGA OLEARIA MEDIUM
1206 CALDANAEROVIRGA MEDIUM
1232 THERMOTOGA TF(D) MEDIUM
1234 ACIDITHIOBACILLUS FERRIVORANS MEDIUM
1236 SPOROLITUUS MEDIUM
1237 TEPIDIMICROBIUM MEDIUM
1243 DESULFITOBACTERIUM AROMATICIVORANS MEDIUM
1251 CALDINITRATIRUPTOR MEDIUM
1254 NAUTILIA NITRATIREDUCENS MEDIUM
1263 SPIROCHAETA DISSIPATITROPHA MEDIUM
1267 FERRIPHASELUS (ES) MEDIUM
1273 METHANOSPIRILLUM LACUNAE MEDIUM
1278 ARCHAEOGLOBUS SULFATICALLIDUS MEDIUM
1290 ALKALIPHILUS HALOPHILUS MEDIUM
1292 CALDICOPROBACTER ALGERIENSIS MEDIUM
1484 Glycerol-Yeast extract-Peptone Medium
1525 KALLOTENUE MEDIUM
1526e LIMIHALOGLOBUS MEDIUM
1526 SALINISPIRA (L21 LS) MEDIUM
1526d LIMISALSIVIBRIO (L21 LS) MEDIUM
1526c DESULFOHALOVIBRIO (L21 LS) MEDIUM
1526a KIRITIMATIELLA (L21 LS) MEDIUM
1527a SALINIVIRGA (L21 HS) MEDIUM
1527 SEDIMENTISPHAERA (L21 HS) MEDIUM
1565 ALKALIPHILUS TRANSVAALENSIS MEDIUM
1570 THIOGRANUM MEDIUM
1577 SPOROHALOBACTER SALINUS MEDIUM
1636 TEPIDIBACILLUS MEDIUM
1662 ALKALIPHILUS HYDROTHERMALIS MEDIUM
1681 Mineral salt medium with colloidal chitin
1699 SULFURIMONAS CRATERIS MEDIUM
1717 PERLABENTIMONAS MEDIUM
1731 PONTIELLA MEDIUM
1786 Geothrix fermentans medium