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DSMZ

DSMZ 1114 . THIOFABA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1114 #

ml
Equipment needed: Gassing station Serum bottle Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 972 ml - -
Solution B 10 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
Solution E 1 ml - -
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into serum vials (to 20% of volume) and autoclave. Solution B is autoclaved separately under 100% N2 gas and solution C under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. The pH of the complete medium should be 6.5.
2 After inoculation cultivation vials are pressurized with an amount of sterile air that is equivalent to 30 vol% of the cultivation vial.

Solution A #

972
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 0.75 g 0.772 3.795
CaCl2 x 2 H2O 0.15 g 0.154 1.05
NH4Cl 0.54 g 0.556 10.386
Modified Wolin's mineral solution 2.00 ml - -
Distilled water 970.00 ml - -

Solution B #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.19 g 119 874.453
K2HPO4 0.21 g 21 120.568
Distilled water 10.00 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 0.5 g 50 471.752
Distilled water 10.0 ml - -

Solution D #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 1.25 g 125 503.655
Distilled water 10.00 ml - -

Solution E #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution (10x) 1 ml - -

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 18.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Gassing station, Serum bottle
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Gas composition

  • 80 % N2
  • 15 % CO2
  • 5 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 1.246 5.021
KH2PO4 1.186 8.718
MgCl2 x 6 H2O 0.748 3.678
NH4Cl 0.538 10.065
Na2CO3 0.499 4.703
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
oxygen microaerophile 1 strain
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