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284. KOSMOTOGA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.14 g 0.137 0.787
2 0.14 g 0.137 0.933
3 0.25 g 0.245 4.578
4 3.40 g 3.33 13.511
5 4.10 g 4.016 19.752
6 0.33 g 0.323 4.335
7
(0.1% w/v)
0.50 ml 4.9e-4 0.002
8 30.00 g 29.383 502.767
9 0.01 g 0.01 0.025
10 Trace element solution 10.00 ml - -
11
(0.1% w/v)
0.50 ml 4.9e-4 0.002
12 1.50 g 1.469 13.862
13 1.00 g 0.979 -
14 Vitamin solution 10.00 ml - -
15 0.50 g 0.49 2.788
16 0.50 g 0.49 2.039
17 1000.00 ml 977.676 54269.2
Instructions:
  1. Dissolve ingredients except carbonate, yeast extract, vitamins, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes and autoclave. Add vitamins (sterilized by filtration), yeast extract, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use check pH of completed medium and adjust to 7.0 - 7.2, if necessary. After inoculation add sterile 80% N2 and 20% CO2 gas mixture to 1 bar overpressure.
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.229 55354.6
NaCl 29.393 502.935
MgCl2 x 6 H2O 4.016 19.752
MgSO4 x 7 H2O 3.359 13.63
Na2CO3 1.469 13.862
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain

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